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Title: | Carriers for metal complexes on tumour cells: the effect of cyclodextrins vs CNTs on the model guest phenanthroline-5,6-dione trithiacyclononane ruthenium(II) chloride | Authors: | Braga, Susana S. Marques, Joana Heister, Elena Diogo, Cátia V. Oliveira, Paulo J. Paz, Filipe A. Almeida Santos, Teresa M. Marques, Maria Paula M. |
Keywords: | Antineoplastic Agents; Cell Survival; Coordination Complexes; Crystallography, X-Ray; Cyclodextrins; DNA; Drug Carriers; Drug Screening Assays, Antitumor; Humans; Inhibitory Concentration 50; MCF-7 Cells; Melanoma; Mice; Models, Molecular; Molecular Conformation; Myoblasts, Cardiac; Nanocapsules; Nanotubes, Carbon; Powder Diffraction; Rats; Thermogravimetry; Transition Temperature | Issue Date: | 1-Jan-2014 | Serial title, monograph or event: | BioMetals | Volume: | 27 | Issue: | 3 | Abstract: | The complex [Ru[9]aneS3(pdon)Cl]Cl (pdon = 1,10-phenanthroline-5,6-dione) was readily obtained from the stoichiometric reaction of Ru[9]aneS3(dmso)Cl2 with pdon. Recrystallisation in ethanol using salicylic acid as a co-crystallisation helper afforded single-crystals suitable for the collection of X-ray diffraction data which afforded a reasonable structural description. Two different kinds of molecular carriers were tested as vehicles for this complex: carbon nanotubes (CNTs) and cyclodextrins. CNTs had an insufficient loading rate for the ruthenium complex at CNT concentrations deemed non-cytotoxic on cultured cells. The cyclodextrin (CD) carriers, β-CD and TRIMEB (standing for permethylated β-CD), were able to form two adducts, studied by powder X-ray diffraction, thermogravimetric analysis (TGA), (13)C{(1)H} CP/MAS NMR and FT-IR spectroscopies. The DNA thermal denaturation studies showed that the complex 1 is able to intercalate with DNA. The in vitro cytotoxicity of the free complex [Ru[9]aneS3(pdon)Cl]Cl (1) and of its two CD adducts (2 and 3) was assessed on both rodent and human cell lines. By using the mouse K1735-M2 melanoma cell line and the non-tumour rat H9c2 cardiomyoblasts, the results showed that 1 and 2 significantly inhibited the growth of the tumour cell line while displaying a good safety profile on cardiomyoblasts. Compound 3 at 100 μM inhibited the proliferation of both cell lines, with a higher activity towards the melanoma cell line. The cytotoxicity of the compounds 1-3 was further assessed on human breast cancer cell lines. Against the MDA-MB-231 line, growth inhibition occurred only with 1 and 3 at the incubation time of 96 h, both with approximate inhibition rates of 50 %; against the MCF-7 line, mild cytotoxicity was observed at 48 h of incubation, with IC50 values calculated above 100 μM for 1, 2 and 3. | URI: | https://hdl.handle.net/10316/45046 | DOI: | 10.1007/s10534-014-9725-8 10.1007/s10534-014-9725-8 |
Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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Biometals_14.pdf | 2.98 MB | Adobe PDF | View/Open |
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