Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/45046
DC Field | Value | Language |
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dc.contributor.author | Braga, Susana S. | - |
dc.contributor.author | Marques, Joana | - |
dc.contributor.author | Heister, Elena | - |
dc.contributor.author | Diogo, Cátia V. | - |
dc.contributor.author | Oliveira, Paulo J. | - |
dc.contributor.author | Paz, Filipe A. Almeida | - |
dc.contributor.author | Santos, Teresa M. | - |
dc.contributor.author | Marques, Maria Paula M. | - |
dc.date.accessioned | 2017-12-15T16:17:55Z | - |
dc.date.available | 2017-12-15T16:17:55Z | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier.uri | https://hdl.handle.net/10316/45046 | - |
dc.description.abstract | The complex [Ru[9]aneS3(pdon)Cl]Cl (pdon = 1,10-phenanthroline-5,6-dione) was readily obtained from the stoichiometric reaction of Ru[9]aneS3(dmso)Cl2 with pdon. Recrystallisation in ethanol using salicylic acid as a co-crystallisation helper afforded single-crystals suitable for the collection of X-ray diffraction data which afforded a reasonable structural description. Two different kinds of molecular carriers were tested as vehicles for this complex: carbon nanotubes (CNTs) and cyclodextrins. CNTs had an insufficient loading rate for the ruthenium complex at CNT concentrations deemed non-cytotoxic on cultured cells. The cyclodextrin (CD) carriers, β-CD and TRIMEB (standing for permethylated β-CD), were able to form two adducts, studied by powder X-ray diffraction, thermogravimetric analysis (TGA), (13)C{(1)H} CP/MAS NMR and FT-IR spectroscopies. The DNA thermal denaturation studies showed that the complex 1 is able to intercalate with DNA. The in vitro cytotoxicity of the free complex [Ru[9]aneS3(pdon)Cl]Cl (1) and of its two CD adducts (2 and 3) was assessed on both rodent and human cell lines. By using the mouse K1735-M2 melanoma cell line and the non-tumour rat H9c2 cardiomyoblasts, the results showed that 1 and 2 significantly inhibited the growth of the tumour cell line while displaying a good safety profile on cardiomyoblasts. Compound 3 at 100 μM inhibited the proliferation of both cell lines, with a higher activity towards the melanoma cell line. The cytotoxicity of the compounds 1-3 was further assessed on human breast cancer cell lines. Against the MDA-MB-231 line, growth inhibition occurred only with 1 and 3 at the incubation time of 96 h, both with approximate inhibition rates of 50 %; against the MCF-7 line, mild cytotoxicity was observed at 48 h of incubation, with IC50 values calculated above 100 μM for 1, 2 and 3. | por |
dc.language.iso | eng | por |
dc.rights | openAccess | por |
dc.subject | Antineoplastic Agents | por |
dc.subject | Cell Survival | por |
dc.subject | Coordination Complexes | por |
dc.subject | Crystallography, X-Ray | por |
dc.subject | Cyclodextrins | por |
dc.subject | DNA | por |
dc.subject | Drug Carriers | por |
dc.subject | Drug Screening Assays, Antitumor | por |
dc.subject | Humans | por |
dc.subject | Inhibitory Concentration 50 | por |
dc.subject | MCF-7 Cells | por |
dc.subject | Melanoma | por |
dc.subject | Mice | por |
dc.subject | Models, Molecular | por |
dc.subject | Molecular Conformation | por |
dc.subject | Myoblasts, Cardiac | por |
dc.subject | Nanocapsules | por |
dc.subject | Nanotubes, Carbon | por |
dc.subject | Powder Diffraction | por |
dc.subject | Rats | por |
dc.subject | Thermogravimetry | por |
dc.subject | Transition Temperature | por |
dc.title | Carriers for metal complexes on tumour cells: the effect of cyclodextrins vs CNTs on the model guest phenanthroline-5,6-dione trithiacyclononane ruthenium(II) chloride | por |
dc.type | article | - |
degois.publication.firstPage | 507 | por |
degois.publication.lastPage | 525 | por |
degois.publication.issue | 3 | por |
degois.publication.title | BioMetals | por |
dc.peerreviewed | yes | por |
dc.identifier.doi | 10.1007/s10534-014-9725-8 | - |
dc.identifier.doi | 10.1007/s10534-014-9725-8 | por |
degois.publication.volume | 27 | por |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | QFM-UC – Molecular Physical-Chemistry R&D Unit | - |
crisitem.author.orcid | 0000-0002-7333-9158 | - |
crisitem.author.orcid | 0000-0002-5201-9948 | - |
crisitem.author.orcid | 0000-0002-8391-0055 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Biometals_14.pdf | 2.98 MB | Adobe PDF | View/Open |
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