Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/27908
Título: | The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy | Autor: | Krzykawska-Serda, Martyna Dąbrowski, Janusz M. Arnaut, Luis G. Szczygieł, Małgorzata Urbańska, Krystyna Stochel, Grażyna Elas, Martyna |
Palavras-chave: | Oxymetry; Blood flow; Vascular-targeted PDT; Bacteriochlorins; Hydroxyl radical; Singlet oxygen; Superoxide; Phototoxicity; Photodynamic therapy; Free radicals | Data: | Ago-2014 | Editora: | Elsevier | Citação: | KRZYKAWSKA-SERDA, Martyna [et. al] - The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. "Free Radical Biology and Medicine". ISSN 0891-5849. Vol. 73 (2014) p. 239–251 | Título da revista, periódico, livro ou evento: | Free Radical Biology and Medicine | Volume: | 73 | Resumo: | Blood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition. | URI: | https://hdl.handle.net/10316/27908 | ISSN: | 0891-5849 | DOI: | 10.1016/j.freeradbiomed.2014.05.003 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Química - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
The role of strong hypoxia in tumors after treatment in the outcome.pdf | 4.3 MB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
74
Visto em 14/out/2024
Citações WEB OF SCIENCETM
5
73
Visto em 2/out/2024
Visualizações de página 50
619
Visto em 15/out/2024
Downloads 50
628
Visto em 15/out/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.