Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/27908
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dc.contributor.authorKrzykawska-Serda, Martyna-
dc.contributor.authorDąbrowski, Janusz M.-
dc.contributor.authorArnaut, Luis G.-
dc.contributor.authorSzczygieł, Małgorzata-
dc.contributor.authorUrbańska, Krystyna-
dc.contributor.authorStochel, Grażyna-
dc.contributor.authorElas, Martyna-
dc.date.accessioned2014-12-16T10:37:29Z-
dc.date.available2014-12-16T10:37:29Z-
dc.date.issued2014-08-
dc.identifier.citationKRZYKAWSKA-SERDA, Martyna [et. al] - The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. "Free Radical Biology and Medicine". ISSN 0891-5849. Vol. 73 (2014) p. 239–251por
dc.identifier.issn0891-5849-
dc.identifier.urihttps://hdl.handle.net/10316/27908-
dc.description.abstractBlood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectOxymetrypor
dc.subjectBlood flowpor
dc.subjectVascular-targeted PDTpor
dc.subjectBacteriochlorinspor
dc.subjectHydroxyl radicalpor
dc.subjectSinglet oxygenpor
dc.subjectSuperoxidepor
dc.subjectPhototoxicitypor
dc.subjectPhotodynamic therapypor
dc.subjectFree radicalspor
dc.titleThe role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapypor
dc.typearticlepor
degois.publication.firstPage239por
degois.publication.lastPage251por
degois.publication.titleFree Radical Biology and Medicinepor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S089158491400210Xpor
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.freeradbiomed.2014.05.003-
degois.publication.volume73por
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-3223-4819-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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