Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/12629
Título: | Partial fusion activity of influenza virus toward liposomes and erythrocyte ghosts is distinct from viral inactivation | Autor: | Ramalho-Santos, João Lima, Maria C. Pedroso de Nir, Shlomo |
Data: | 27-Set-1996 | Editora: | The American Society for Biochemistry and Molecular Biology | Citação: | The Journal of Biological Chemistry. 271:39 (1996) 23902-23906 | Resumo: | Final extents of fusion of influenza virus (A/PR/8/34 strain) with neutral and partially acidic liposomes were monitored with (i) a fluorescence resonance energy-transfer assay in which the liposomes were labeled and (ii) by the dequenching of octadecylrhodamine, initially incorporated in the viral membrane. The latter assay was also employed in the fusion of influenza virus and Sendai virus with erythrocyte ghosts. In all cases, a phenomenon of partial fusion activity of the virus was observed, which is distinct from low pH inactivation. The unfused influenza or Sendai virions, which were separated by sucrose gradient centrifugation from liposomes or erythrocyte ghosts exhibited again partial fusion activity toward freshly added liposomes or ghosts, respectively. The conclusion is that the fraction of initially bound and unfused virions does not consist of defective particles, but rather of particles bound to the target membranes via inactive sites on the virus (or on cellular membranes), or else, partial fusion activity is a manifestation of a certain probability of production of fusion inactive sites by irreversible association of viral glycoproteins or peptides in the target membrane | URI: | https://hdl.handle.net/10316/12629 | ISSN: | 0021-9258 | DOI: | 10.1074/jbc.271.39.23902 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Partial fusion activity of influenza virus.pdf | 188.71 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
15
Visto em 9/nov/2022
Citações WEB OF SCIENCETM
13
Visto em 2/mai/2023
Visualizações de página
297
Visto em 7/ago/2024
Downloads 50
679
Visto em 7/ago/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.