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Title: Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
Authors: Cruz, M. Teresa 
Gonçalo, Margarida 
Figueiredo, Américo 
Carvalho, Arsélio P. 
Duarte, Carlos B. 
Lopes, M. Celeste 
Keywords: NO; iNOS; Skin dendritic cell; Contact sensitizer; Nickel sulfate; NF-kB; AP-1
Issue Date: 2004
Publisher: Blackwell Munksgaard
Citation: Experimental Dermatology. 13 (2004) 18–26
Abstract: Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.
ISSN: 0906-6705
DOI: 10.1016/s0378-4274(03)90098-1
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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