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dc.contributor.authorCruz, M. Teresa-
dc.contributor.authorGonçalo, Margarida-
dc.contributor.authorFigueiredo, Américo-
dc.contributor.authorCarvalho, Arsélio P.-
dc.contributor.authorDuarte, Carlos B.-
dc.contributor.authorLopes, M. Celeste-
dc.identifier.citationExperimental Dermatology. 13 (2004) 18–26en_US
dc.description.abstractNuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.en_US
dc.publisherBlackwell Munksgaarden_US
dc.subjectSkin dendritic cellen_US
dc.subjectContact sensitizeren_US
dc.subjectNickel sulfateen_US
dc.titleContact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell lineen_US
item.fulltextCom Texto completo-
item.languageiso639-1en- of Pharmacy- of Medicine- of Medicine- of Sciences and Technology- of Coimbra- of Coimbra- of Coimbra- - Center for Neuroscience and Cell Biology- - Center for Neuroscience and Cell Biology- - Center for Neuroscience and Cell Biology-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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