Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/11804
Título: | Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line | Autor: | Cruz, M. Teresa Gonçalo, Margarida Figueiredo, Américo Carvalho, Arsélio P. Duarte, Carlos B. Lopes, M. Celeste |
Palavras-chave: | NO; iNOS; Skin dendritic cell; Contact sensitizer; Nickel sulfate; NF-kB; AP-1 | Data: | 2004 | Editora: | Blackwell Munksgaard | Citação: | Experimental Dermatology. 13 (2004) 18–26 | Resumo: | Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells. | URI: | https://hdl.handle.net/10316/11804 | ISSN: | 0906-6705 | DOI: | 10.1016/s0378-4274(03)90098-1 | Direitos: | openAccess |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Contact sensitizer nickel sulfate.pdf | 877.61 kB | Adobe PDF | Ver/Abrir |
Visualizações de página
340
Visto em 16/abr/2024
Downloads 20
971
Visto em 16/abr/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.