Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/111794
Title: The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
Authors: Rodrigues, Elsa T. 
Pereira, Eduarda 
Oliveira, Paulo J. 
Pardal, Miguel Ângelo 
Keywords: Effluent toxicity testing; Environmental biomonitoring; Environmental health indicators; Temporal variability; H9c2(2-1) cells; Sulforhodamine B assay
Issue Date: 31-Oct-2023
Publisher: Springer Nature
Project: FCT CEECIND/03543/2017 
UIDB/04004/2020 
Associate Laboratory TERRA (LA/P/0092/2020) Strategic Projects 
Serial title, monograph or event: Environmental Monitoring and Assessment
Volume: 195
Issue: 11
Abstract: The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To correlate standard analytical method results with in vitro results, a total of 16 physicochemical parameters, such as nutrients, pH, chemical oxygen demand, total suspended solids and metals, were determined in both raw and treated wastewater samples. Results revealed that the H9c2(2-1) cell-based SRB assay has an enormous potential to evaluate municipal wastewater quality over time and to discriminate influent and effluent toxic characteristics, as well as for water quality monitoring and surveillance of the efficacy of treatment processes. Finally, the gathered results alerted to the impact of phosphates in a biological system, leading us to recommend the selection of this parameter as a potential environmental health indicator.
URI: https://hdl.handle.net/10316/111794
ISSN: 0167-6369
1573-2959
DOI: 10.1007/s10661-023-12017-8
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais

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