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Title: Metabolic effects of hypoxia in colorectal cancer by 13C NMR isotopomer analysis
Authors: Abrantes, Ana M. 
Tavares, Ludgero C. 
Pires, Salomé 
Casalta-Lopes, João 
Mendes, Cândida 
Simões, Marta 
Grazina, Manuela M. 
Carvalho, Rui A. 
Botelho, Maria Filomena 
Issue Date: 2014
Publisher: Hindawi
Project: PTDC/EBBEBI/ 115810/2009 
Centre of Investigation on Environment Genetics and Oncobiology (CIMAGO) 
Serial title, monograph or event: BioMed Research International
Volume: 2014
Abstract: (13)C NMR isotopomer analysis was used to characterize intermediary metabolism in three colorectal cancer cell lines (WiDr, LS1034, and C2BBe1) and determine the "metabolic remodeling" that occurs under hypoxia. Under normoxia, the three colorectal cancer cell lines present high rates of lactate production and can be seen as "Warburg" like cancer cells independently of substrate availability, since such profile was dominant at both high and low glucose media contents. The LS1034 was the less glycolytic of the three cell lines and was the most affected by the event of hypoxia, raising abruptly glucose consumption and lactate production. The other two colorectal cell lines, WiDr and C2BBe1, adapted better to hypoxia and were able to maintain their oxidative fluxes even at the very low levels of oxygen. These differential metabolic behaviors of the three colorectal cell lines show how important an adequate knowledge of the "metabolic remodeling" that follows a given cancer treatment is towards the correct (re)design of therapeutic strategies against cancer.
ISSN: 2314-6133
DOI: 10.1155/2014/759791
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais

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