Utilize este identificador para referenciar este registo: https://hdl.handle.net/10316/107135
Título: Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization
Autor: Rodrigues, Dina
Costa-Pinto, Ana R.
Sousa, Sérgio
Vasconcelos, Marta W.
Pintado, Manuela M.
Pereira, Leonel 
Rocha-Santos, Teresa A. P.
Costa, João P. da
Silva, Artur M. S.
Duarte, Armando C.
Gomes, Ana M. P.
Freitas, Ana C.
Palavras-chave: Osmundea pinnatifida; Sargassum muticum; enzymatic extracts; minerals; mono and polysaccharides; FTIR-ATR; NMR; cytotoxicity
Data: 3-Abr-2019
Editora: MDPI
Projeto: This work was supported by national funds through Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Educação (MEC) (Programa de Investimentos e Despesas de Desenvolvimento da Administração Central—PIDDAC), through project references IF/00588/2015 and IF/00407/2013/CP1162/CT0023 as well by Fundo Europeu de Desenvolvimento Regional (FEDER) through project NORTE-01-0145-FEDER-000012 (Structured Programme on Bioengineering Therapies for Infectious) funded by NORTE2020 program. This work was also supported by the Fundação para a Ciência e a Tecnologia (FCT), through the strategic project UID/MAR/04292/2019 granted to MARE. Thanks, are also due, for the financial support, to CESAM (UID/AMB/50017) through FCT/MEC national funds. 
Título da revista, periódico, livro ou evento: Marine Drugs
Volume: 17
Número: 4
Resumo: Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.
URI: https://hdl.handle.net/10316/107135
ISSN: 1660-3397
DOI: 10.3390/md17040209
Direitos: openAccess
Aparece nas coleções:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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