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Título: | Cofilin-1 Is a Mechanosensitive Regulator of Transcription | Autor: | Domingues, Catarina Geraldo, A. Margarida Anjo, Sandra I. Matos, André Almeida, Cláudio Caramelo, Inês Lopes-da-Silva, José A Paiva, Artur Carvalho, João Neves, Ricardo Pires das Manadas, Bruno Grãos, Mário |
Palavras-chave: | mechanotransduction; Cofilin-1; cytoskeleton; transcription; hUCM-MSCs; proteomics | Data: | 2020 | Editora: | Frontiers Media S.A. | Projeto: | POCI-01- 0145-FEDER-029516 (ref.: PTDC/MED-NEU/29516/2017) POCI-01-0145-FEDER-029311 (ref.: PTDC/BTMTEC/ 29311/2017) POCI-01-0145-FEDER-016428 (ref.: SAICTPAC/0010/2015) UID/NEU/04539/2019 (CNC.IBILI strategic plan) UIDB/04539/2020 (CIBB strategic plan) |
Título da revista, periódico, livro ou evento: | Frontiers in Cell and Developmental Biology | Volume: | 8 | Resumo: | The mechanical properties of the extracellular environment are interrogated by cells and integrated through mechanotransduction. Many cellular processes depend on actomyosin-dependent contractility, which is influenced by the microenvironment's stiffness. Here, we explored the influence of substrate stiffness on the proteome of proliferating undifferentiated human umbilical cord-matrix mesenchymal stem/stromal cells. The relative abundance of several proteins changed significantly by expanding cells on soft (∼3 kPa) or stiff substrates (GPa). Many such proteins are associated with the regulation of the actin cytoskeleton, a major player of mechanotransduction and cell physiology in response to mechanical cues. Specifically, Cofilin-1 levels were elevated in cells cultured on soft comparing with stiff substrates. Furthermore, Cofilin-1 was de-phosphorylated (active) and present in the nuclei of cells kept on soft substrates, in contrast with phosphorylated (inactive) and widespread distribution in cells on stiff. Soft substrates promoted Cofilin-1-dependent increased RNA transcription and faster RNA polymerase II-mediated transcription elongation. Cofilin-1 is part of a novel mechanism linking mechanotransduction and transcription. | URI: | https://hdl.handle.net/10316/106102 | ISSN: | 2296-634X | DOI: | 10.3389/fcell.2020.00678 | Direitos: | openAccess |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais I&D CFis - Artigos em Revistas Internacionais I&D CFis - Artigos em Revistas Internacionais |
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Cofilin-1 Is a Mechanosensitive Regulator of Transcription.pdf | 4.36 MB | Adobe PDF | Ver/Abrir |
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