Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106102
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Domingues, Catarina | - |
dc.contributor.author | Geraldo, A. Margarida | - |
dc.contributor.author | Anjo, Sandra I. | - |
dc.contributor.author | Matos, André | - |
dc.contributor.author | Almeida, Cláudio | - |
dc.contributor.author | Caramelo, Inês | - |
dc.contributor.author | Lopes-da-Silva, José A | - |
dc.contributor.author | Paiva, Artur | - |
dc.contributor.author | Carvalho, João | - |
dc.contributor.author | Neves, Ricardo Pires das | - |
dc.contributor.author | Manadas, Bruno | - |
dc.contributor.author | Grãos, Mário | - |
dc.date.accessioned | 2023-03-20T13:34:35Z | - |
dc.date.available | 2023-03-20T13:34:35Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 2296-634X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106102 | - |
dc.description.abstract | The mechanical properties of the extracellular environment are interrogated by cells and integrated through mechanotransduction. Many cellular processes depend on actomyosin-dependent contractility, which is influenced by the microenvironment's stiffness. Here, we explored the influence of substrate stiffness on the proteome of proliferating undifferentiated human umbilical cord-matrix mesenchymal stem/stromal cells. The relative abundance of several proteins changed significantly by expanding cells on soft (∼3 kPa) or stiff substrates (GPa). Many such proteins are associated with the regulation of the actin cytoskeleton, a major player of mechanotransduction and cell physiology in response to mechanical cues. Specifically, Cofilin-1 levels were elevated in cells cultured on soft comparing with stiff substrates. Furthermore, Cofilin-1 was de-phosphorylated (active) and present in the nuclei of cells kept on soft substrates, in contrast with phosphorylated (inactive) and widespread distribution in cells on stiff. Soft substrates promoted Cofilin-1-dependent increased RNA transcription and faster RNA polymerase II-mediated transcription elongation. Cofilin-1 is part of a novel mechanism linking mechanotransduction and transcription. | pt |
dc.language.iso | eng | pt |
dc.publisher | Frontiers Media S.A. | pt |
dc.relation | POCI-01- 0145-FEDER-029516 (ref.: PTDC/MED-NEU/29516/2017) | pt |
dc.relation | POCI-01-0145-FEDER-029311 (ref.: PTDC/BTMTEC/ 29311/2017) | pt |
dc.relation | POCI-01-0145-FEDER-016428 (ref.: SAICTPAC/0010/2015) | pt |
dc.relation | UID/NEU/04539/2019 (CNC.IBILI strategic plan) | pt |
dc.relation | UIDB/04539/2020 (CIBB strategic plan) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | mechanotransduction | pt |
dc.subject | Cofilin-1 | pt |
dc.subject | cytoskeleton | pt |
dc.subject | transcription | pt |
dc.subject | hUCM-MSCs | pt |
dc.subject | proteomics | pt |
dc.title | Cofilin-1 Is a Mechanosensitive Regulator of Transcription | pt |
dc.type | article | - |
degois.publication.firstPage | 678 | pt |
degois.publication.title | Frontiers in Cell and Developmental Biology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3389/fcell.2020.00678 | pt |
degois.publication.volume | 8 | pt |
dc.date.embargo | 2020-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CFisUC – Center for Physics of the University of Coimbra | - |
crisitem.author.orcid | 0000-0002-6562-5859 | - |
crisitem.author.orcid | 0000-0002-3015-7821 | - |
crisitem.author.orcid | 0000-0002-2087-4042 | - |
crisitem.author.orcid | 0000-0002-2707-1488 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais I&D CFis - Artigos em Revistas Internacionais I&D CFis - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cofilin-1 Is a Mechanosensitive Regulator of Transcription.pdf | 4.36 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
6
checked on May 13, 2024
WEB OF SCIENCETM
Citations
6
checked on May 2, 2024
Page view(s)
116
checked on May 7, 2024
Download(s)
67
checked on May 7, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License