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Título: | Immobilization of Streptavidin on a Plasmonic Au-TiO2 Thin Film towards an LSPR Biosensing Platform | Autor: | Pereira-Silva, Patrícia Meira, Diana I. Costa-Barbosa, Augusto Costa, Diogo Rodrigues, Marco S. Borges, Joel Machado, Ana V. Cavaleiro, Albano Sampaio, Paula Vaz, Filipe |
Palavras-chave: | Au-TiO2 thin film; plasmonic biosensor; streptavidin–biotin; LSPR detection | Data: | 1-Mai-2022 | Editora: | MDPI | Projeto: | 04650/2020 UIDB/04050/2020 UID/EMS/00285/2020 POCI-01-0145-FEDER-032299 info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.08235.BD/PT/Development of nanocomposite ZnO thin films with antibiofilm and antimicrobial properties to prevent pathogens’ transmission SFRH/BD/136279/2018 COVID/BD/ 152169/2021 info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH/BD/133513/2017/PT/New generation antigen delivery system for Candida albicans recombinant proteins as a vaccination strategy against systemic fungal infections info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC/FIS-MAC/32299/2017/PT/Nanopartículas plasmónicas para deteção biológica info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH/BD/143262/2019/PT/Development of nanoplasmonic thin film biosensors with enhanced sensitivity for detection of Ochratoxin-A |
Título da revista, periódico, livro ou evento: | Nanomaterials | Volume: | 12 | Número: | 9 | Resumo: | Optical biosensors based on localized surface plasmon resonance (LSPR) are the future of label-free detection methods. This work reports the development of plasmonic thin films, containing Au nanoparticles dispersed in a TiO2 matrix, as platforms for LSPR biosensors. Post-deposition treatments were employed, namely annealing at 400 °C, to develop an LSPR band, and Ar plasma, to improve the sensitivity of the Au-TiO2 thin film. Streptavidin and biotin conjugated with horseradish peroxidase (HRP) were chosen as the model receptor-analyte, to prove the efficiency of the immobilization method and to demonstrate the potential of the LSPR-based biosensor. The Au-TiO2 thin films were activated with O2 plasma, to promote the streptavidin immobilization as a biorecognition element, by increasing the surface hydrophilicity (contact angle drop to 7°). The interaction between biotin and the immobilized streptavidin was confirmed by the detection of HRP activity (average absorbance 1.9 ± 0.6), following a protocol based on enzyme-linked immunosorbent assay (ELISA). Furthermore, an LSPR wavelength shift was detectable (0.8 ± 0.1 nm), resulting from a plasmonic thin-film platform with a refractive index sensitivity estimated to be 33 nm/RIU. The detection of the analyte using these two different methods proves that the functionalization protocol was successful and the Au-TiO2 thin films have the potential to be used as an LSPR platform for label-free biosensors. | URI: | https://hdl.handle.net/10316/103426 | ISSN: | 2079-4991 | DOI: | 10.3390/nano12091526 | Direitos: | openAccess |
Aparece nas coleções: | I&D CEMMPRE - Artigos em Revistas Internacionais |
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Immobilization-of-Streptavidin-on-a-Plasmonic-AuTiO2-Thin-Film-towards-an-LSPR-Biosensing-PlatformNanomaterials.pdf | 7.54 MB | Adobe PDF | Ver/Abrir |
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