Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103426
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pereira-Silva, Patrícia | - |
dc.contributor.author | Meira, Diana I. | - |
dc.contributor.author | Costa-Barbosa, Augusto | - |
dc.contributor.author | Costa, Diogo | - |
dc.contributor.author | Rodrigues, Marco S. | - |
dc.contributor.author | Borges, Joel | - |
dc.contributor.author | Machado, Ana V. | - |
dc.contributor.author | Cavaleiro, Albano | - |
dc.contributor.author | Sampaio, Paula | - |
dc.contributor.author | Vaz, Filipe | - |
dc.date.accessioned | 2022-11-11T10:01:59Z | - |
dc.date.available | 2022-11-11T10:01:59Z | - |
dc.date.issued | 2022-05-01 | - |
dc.identifier.issn | 2079-4991 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103426 | - |
dc.description.abstract | Optical biosensors based on localized surface plasmon resonance (LSPR) are the future of label-free detection methods. This work reports the development of plasmonic thin films, containing Au nanoparticles dispersed in a TiO2 matrix, as platforms for LSPR biosensors. Post-deposition treatments were employed, namely annealing at 400 °C, to develop an LSPR band, and Ar plasma, to improve the sensitivity of the Au-TiO2 thin film. Streptavidin and biotin conjugated with horseradish peroxidase (HRP) were chosen as the model receptor-analyte, to prove the efficiency of the immobilization method and to demonstrate the potential of the LSPR-based biosensor. The Au-TiO2 thin films were activated with O2 plasma, to promote the streptavidin immobilization as a biorecognition element, by increasing the surface hydrophilicity (contact angle drop to 7°). The interaction between biotin and the immobilized streptavidin was confirmed by the detection of HRP activity (average absorbance 1.9 ± 0.6), following a protocol based on enzyme-linked immunosorbent assay (ELISA). Furthermore, an LSPR wavelength shift was detectable (0.8 ± 0.1 nm), resulting from a plasmonic thin-film platform with a refractive index sensitivity estimated to be 33 nm/RIU. The detection of the analyte using these two different methods proves that the functionalization protocol was successful and the Au-TiO2 thin films have the potential to be used as an LSPR platform for label-free biosensors. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | 04650/2020 | pt |
dc.relation | UIDB/04050/2020 | pt |
dc.relation | UID/EMS/00285/2020 | pt |
dc.relation | POCI-01-0145-FEDER-032299 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.08235.BD/PT/Development of nanocomposite ZnO thin films with antibiofilm and antimicrobial properties to prevent pathogens’ transmission | pt |
dc.relation | SFRH/BD/136279/2018 | pt |
dc.relation | COVID/BD/ 152169/2021 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH/BD/133513/2017/PT/New generation antigen delivery system for Candida albicans recombinant proteins as a vaccination strategy against systemic fungal infections | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC/FIS-MAC/32299/2017/PT/Nanopartículas plasmónicas para deteção biológica | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH/BD/143262/2019/PT/Development of nanoplasmonic thin film biosensors with enhanced sensitivity for detection of Ochratoxin-A | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Au-TiO2 thin film | pt |
dc.subject | plasmonic biosensor | pt |
dc.subject | streptavidin–biotin | pt |
dc.subject | LSPR detection | pt |
dc.title | Immobilization of Streptavidin on a Plasmonic Au-TiO2 Thin Film towards an LSPR Biosensing Platform | pt |
dc.type | article | - |
degois.publication.firstPage | 1526 | pt |
degois.publication.issue | 9 | pt |
degois.publication.title | Nanomaterials | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/nano12091526 | pt |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2022-05-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Physics Center of Minho and Porto Universities | - |
crisitem.project.grantno | CENTRE OF MOLECULAR AND ENVIRONMENTAL BIOLOGY | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0001-7421-6902 | - |
Appears in Collections: | I&D CEMMPRE - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Immobilization-of-Streptavidin-on-a-Plasmonic-AuTiO2-Thin-Film-towards-an-LSPR-Biosensing-PlatformNanomaterials.pdf | 7.54 MB | Adobe PDF | View/Open |
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