Ultrastructure and cytochemistry of Eucalyptus globulus(Myrtaceae) pollen grain

Abstract

The morphology, ultrastructure and cytochemistry of Eucalyptus globulusmature pollen were investigated using light (LM), scanning electron (SEM) and transmission electron microscopy (TEM). The pollen morphology is typically myrtaceous with a suite of characters that allows its distinction from the pollen of other Eucalyptusspecies. The exine consists of a thick endexine and a massive ectexine with hardly distinguishable columellae. The endexine is 2-layered towards the apertural regions where the inner and spongy-granulate layer forms a continuous colpus membrane and a thinner pore membrane. Under the pores the intine is 3-layered forming complex onci. The spindle-shaped generative cell (GC) is deeply undulated and located in a cup-shaped depression of the vegetative cell (VC) nucleus. In the dense VC cytoplasm the main storage reserves are lipid bodies and insoluble carbohydrates in the cytosol, although proteins are also present. The most characteristic feature of the VC cytoplasm is the extremely well-developed rough endoplasmic reticulum (RER), which forms extensive stacks filling large areas of the central cytoplasm. Single RER cisternae are also scattered throughout the cytoplasm, most of them establishing an intimate association with lipid bodies, storage vacuoles, the VC plasmalemma and a few proplastids. The physiological significance of the RER stacks and of the RER cisternae association with other cell components, as well as the structure and function of an endomembrane compartment, found only in the freeze-fixed pollen, are discussed.