Please use this identifier to cite or link to this item:
|Title:||Calcium-dependent mitochondrial permeability transition is augmented in the kidney of Goto-Kakizaki diabetic rat||Authors:||Oliveira, Paulo J.
Esteves, Telma C.
Moreno, António J. M.
Santos, Maria S.
|Issue Date:||2004||Citation:||Diabetes/Metabolism Research and Reviews. 20:2 (2004) 131-136||Abstract:||Renal disease associated with diabetes mellitus is a major problem among diabetic patients. The role of mitochondria in the pathogenesis of diabetes has received a large amount of attention in the last years, but many aspects of this subject are still poorly understood. In the present study, we studied the susceptibility of the mitochondrial permeability transition (MPT) on kidney mitochondria from the Goto-Kakizaki (GK) rat, an animal model featuring physiological and pathological alterations characteristic of type 2 diabetes.Kidney mitochondria were isolated by differential centrifugations; mitochondrial electric transmembrane potential and calcium loading capacity were evaluated with a TPP+-selective electrode and with a calcium-sensitive fluorescent probe. Coenzyme Q9, Q10 and vitamin E were evaluated by high-performance liquid chromatography (HPLC).Kidney mitochondria from the diabetic animals had an increased susceptibility to the induction of the MPT by calcium. We observed a loss of calcium-loading capacity and a higher calcium-induced mitochondrial depolarization. Vitamin E and coenzyme Q9 were also increased in kidney mitochondria from GK rats.The results show an enhanced MPT activation in kidney mitochondria from GK rats, which lead us to suggest that this condition may be one major alteration triggered by chronic diabetes in kidney cells, ultimately leading to cell dysfunction. Copyright © 2004 John Wiley & Sons, Ltd.||URI:||http://hdl.handle.net/10316/8342||DOI:||10.1002/dmrr.423||Rights:||openAccess|
|Appears in Collections:||FCTUC Ciências da Vida - Artigos em Revistas Internacionais|
Show full item record
checked on Feb 18, 2020
WEB OF SCIENCETM
checked on Mar 2, 2022
Page view(s) 10867
checked on Aug 4, 2022
checked on Aug 4, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.