Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/2805
Título: | Enantioselective HPLC-UV method for determination of eslicarbazepine acetate (BIA 2-093) and its metabolites in human plasma | Autor: | Alves, Gilberto Figueiredo, Isabel Castel-Branco, Margarida Loureiro, Ana Fortuna, Ana Falcão, Amílcar Caramona, Margarida |
Palavras-chave: | eslicarbazepine acetate; BIA 2-093; oxcarbazepine; human plasma; bioanalytical method validation; enantioselective HPLC-UV method | Data: | 26-Jun-2007 | Editora: | John Wiley & Sons | Citação: | Alves,Gilberto et al. - Enantioselective HPLC-UV method for determination of eslicarbazepine acetate (BIA 2-093) and its metabolites in human plasma. Biomed Chromatogr 21 (2007) 1127–1134 | Projeto: | SFRH/12694/2003 | Título da revista, periódico, livro ou evento: | Biomedical Chromatography | Volume: | 21 | Número: | 11 | Resumo: | Eslicarbazepine acetate (BIA 2-093) is a novel central nervous system drug undergoing clinical phase III trials for epilepsy and phase II trials for bipolar disorder. A simple and reliable chiral reversed-phase HPLC-UV method was developed and validated for the simultaneous determination of eslicarbazepine acetate, oxcarbazepine, S-licarbazepine and R-licarbazepine in human plasma. The analytes and internal standard were extracted from plasma by a solid-phase extraction using Waters Oasis® HLB cartridges. Chromatographic separation was achieved by isocratic elution with water–methanol (88:12, v/v), at a flow rate of 0.7 mL/min, on a LichroCART 250-4 ChiraDex (β-cyclodextrin, 5 μm) column at 30°C. All compounds were detected at 225 nm. Calibration curves were linear over the range 0.4–8 μg/mL for eslicarbazepine acetate and oxcarbazepine, and 0.4 – 80 μg/ mL for each licarbazepine enantiomer. The overall intra- and interday precision and accuracy did not exceed 15%. Mean relative recoveries varied from 94.00 to 102.23% and the limit of quantification of the assay was 0.4 μg/mL for all compounds. This method seems to be a useful tool for clinical research and therapeutic drug monitoring of eslicarbazepine acetate and its metabolites S-licarbazepine, R-licarbazepine and oxcarbazepine. | URI: | https://hdl.handle.net/10316/2805 | DOI: | 10.1002/bmc.858 | Direitos: | openAccess |
Aparece nas coleções: | FFUC- Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Biomedical Chrom.pdf | 214.92 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
31
Visto em 11/nov/2022
Citações WEB OF SCIENCETM
29
Visto em 2/mai/2023
Visualizações de página 50
663
Visto em 16/out/2024
Downloads 10
1.940
Visto em 16/out/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.