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Title: Enantioselective HPLC-UV method for determination of eslicarbazepine acetate (BIA 2-093) and its metabolites in human plasma
Authors: Alves, Gilberto 
Figueiredo, Isabel 
Castel-Branco, Margarida 
Loureiro, Ana 
Fortuna, Ana 
Falcão, Amílcar 
Caramona, Margarida 
Keywords: eslicarbazepine acetate; BIA 2-093; oxcarbazepine; human plasma; bioanalytical method validation; enantioselective HPLC-UV method
Issue Date: 26-Jun-2007
Publisher: John Wiley & Sons
Citation: Alves,Gilberto et al. - Enantioselective HPLC-UV method for determination of eslicarbazepine acetate (BIA 2-093) and its metabolites in human plasma. Biomed Chromatogr 21 (2007) 1127–1134
Abstract: Eslicarbazepine acetate (BIA 2-093) is a novel central nervous system drug undergoing clinical phase III trials for epilepsy and phase II trials for bipolar disorder. A simple and reliable chiral reversed-phase HPLC-UV method was developed and validated for the simultaneous determination of eslicarbazepine acetate, oxcarbazepine, S-licarbazepine and R-licarbazepine in human plasma. The analytes and internal standard were extracted from plasma by a solid-phase extraction using Waters Oasis® HLB cartridges. Chromatographic separation was achieved by isocratic elution with water–methanol (88:12, v/v), at a flow rate of 0.7 mL/min, on a LichroCART 250-4 ChiraDex (β-cyclodextrin, 5 μm) column at 30°C. All compounds were detected at 225 nm. Calibration curves were linear over the range 0.4–8 μg/mL for eslicarbazepine acetate and oxcarbazepine, and 0.4 – 80 μg/ mL for each licarbazepine enantiomer. The overall intra- and interday precision and accuracy did not exceed 15%. Mean relative recoveries varied from 94.00 to 102.23% and the limit of quantification of the assay was 0.4 μg/mL for all compounds. This method seems to be a useful tool for clinical research and therapeutic drug monitoring of eslicarbazepine acetate and its metabolites S-licarbazepine, R-licarbazepine and oxcarbazepine.
DOI: 10.1002/bmc.858
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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