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https://hdl.handle.net/10316/27896
Title: | Short-term exposure of nontumorigenic human bronchial epithelial cells to carcinogenic chromium(VI) compromises their respiratory capacity and alters their bioenergetic signature | Authors: | Cerveira, Joana F. Sánchez-Aragó, María Urbano, Ana M. Cuezva, José M. |
Keywords: | Chromate lung cancer; Warburg effect; Aerobic glycolysis; Cellular respiration; Cellular bioenergetic index; Cellular energy status | Issue Date: | 2014 | Publisher: | Elsevier | Citation: | CERVEIRA, Joana F. [et. al] - Short-term exposure of nontumorigenic human bronchial epithelial cells to carcinogenic chromium(VI) compromises their respiratory capacity and alters their bioenergetic signature. "FEBS Open Bio". ISSN 2211-5463. Vol. 4 (2014) p. 594–601 | Serial title, monograph or event: | FEBS Open Bio | Volume: | 4 | Abstract: | Previous studies on the impact of hexavalent chromium [Cr(VI)] on mammalian cell energetics revealed alterations suggestive of a shift to a more fermentative metabolism. Aiming at a more defined understanding of the metabolic effects of Cr(VI) and of their molecular basis, we assessed the impact of a mild Cr(VI) exposure on critical bioenergetic parameters (lactate production, oxygen consumption and intracellular ATP levels). Cells derived from normal human bronchial epithelium (BEAS-2B cell line), the main in vivo target of Cr(VI) carcinogenicity, were subjected for 48 h to 1 μM Cr(VI). We could confirm a shift to a more fermentative metabolism, resulting from the simultaneous inhibition of respiration and stimulation of glycolysis. This shift was accompanied by a decrease in the protein levels of the catalytic subunit (subunit β) of the mitochondrial H+-ATP synthase (β-F1-ATPase) and a concomitant marked increase in those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The corresponding alteration in the β-F1-ATPase/GAPDH protein ratio (viewed as a bioenergetic signature) upon Cr(VI) exposure was in agreement with the observed attenuation of cellular respiration and enhancement of glycolytic flux. Altogether, these results constitute a novel finding in terms of the molecular mechanisms of Cr(VI) effects. | URI: | https://hdl.handle.net/10316/27896 | ISSN: | 2211-5463 | DOI: | 10.1016/j.fob.2014.06.006 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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