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Title: | Electrochemical evaluation of glutathione S-transferase kinetic parameters | Authors: | Enache, Teodor Adrian Brett, Ana Maria Oliveira |
Keywords: | Glutathione; 1-Chloro-2,4-dinitrobenzene; Glutathione S-transferase; Electrochemistry; Oxidation | Issue Date: | Feb-2015 | Publisher: | Elsevier | Citation: | ENACHE, Teodor Adrian; OLIVEIRA-BRETT, Ana Maria - Electrochemical evaluation of glutathione S-transferase kinetic parameters. "Bioelectrochemistry". ISSN 1567-5394. Vol. 101 (2014) p. 46–51 | Serial title, monograph or event: | Bioelectrochemistry | Volume: | 101 | Abstract: | Glutathione S-transferases (GSTs), are a family of enzymes belonging to the phase II metabolism that catalyse the formation of thioether conjugates between the endogenous tripeptide glutathione and xenobiotic compounds. The voltammetric behaviour of glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione S-transferase (GST), as well as the catalytic conjugation reaction of GSH to CDNB by GST was investigated at room temperature, T = 298.15 K (25 °C), at pH 6.5, for low concentration of substrates and enzyme, using differential pulse (DP) voltammetry at a glassy carbon electrode. Only GSH can be oxidized; a sensitivity of 0.14 nA/μM and a LOD of 6.4 μM were obtained. The GST kinetic parameter electrochemical evaluation, in relation to its substrates, GSH and CDNB, using reciprocal Michaelis–Menten and Lineweaver–Burk double reciprocal plots, was determined. A value of KM ~ 100 μM was obtained for either GSH or CDNB, and Vmax varied between 40 and 60 μmol/min per mg of GST. | URI: | https://hdl.handle.net/10316/27835 | ISSN: | 1567-5394 | DOI: | 10.1016/j.bioelechem.2014.07.002 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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