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Title: Electrochemical evaluation of glutathione S-transferase kinetic parameters
Authors: Enache, Teodor Adrian 
Brett, Ana Maria Oliveira 
Keywords: Glutathione; 1-Chloro-2,4-dinitrobenzene; Glutathione S-transferase; Electrochemistry; Oxidation
Issue Date: Feb-2015
Publisher: Elsevier
Citation: ENACHE, Teodor Adrian; OLIVEIRA-BRETT, Ana Maria - Electrochemical evaluation of glutathione S-transferase kinetic parameters. "Bioelectrochemistry". ISSN 1567-5394. Vol. 101 (2014) p. 46–51
Serial title, monograph or event: Bioelectrochemistry
Volume: 101
Abstract: Glutathione S-transferases (GSTs), are a family of enzymes belonging to the phase II metabolism that catalyse the formation of thioether conjugates between the endogenous tripeptide glutathione and xenobiotic compounds. The voltammetric behaviour of glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione S-transferase (GST), as well as the catalytic conjugation reaction of GSH to CDNB by GST was investigated at room temperature, T = 298.15 K (25 °C), at pH 6.5, for low concentration of substrates and enzyme, using differential pulse (DP) voltammetry at a glassy carbon electrode. Only GSH can be oxidized; a sensitivity of 0.14 nA/μM and a LOD of 6.4 μM were obtained. The GST kinetic parameter electrochemical evaluation, in relation to its substrates, GSH and CDNB, using reciprocal Michaelis–Menten and Lineweaver–Burk double reciprocal plots, was determined. A value of KM ~ 100 μM was obtained for either GSH or CDNB, and Vmax varied between 40 and 60 μmol/min per mg of GST.
ISSN: 1567-5394
DOI: 10.1016/j.bioelechem.2014.07.002
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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