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Title: Chitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healing
Authors: Moura, Liane I. F. 
Dias, Ana M. A. 
Leal, Ermelindo C. 
Carvalho, Lina 
Sousa, Hermínio C. de 
Carvalho, Eugénia 
Keywords: Chitosan derivatives; Wound dressings; Diabetic foot ulcers; Neurotensin; Wound healing
Issue Date: Feb-2014
Publisher: Elsevier
Citation: MOURA, Liane I. F. [et. al] - Chitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healing. "Acta Biomaterialia". ISSN 1742-7061. Vol. 10 Nº. 2 (2014) p. 843-857
Serial title, monograph or event: Acta Biomaterialia
Volume: 10
Issue: 2
Abstract: One important complication of diabetes mellitus is chronic, non-healing diabetic foot ulcers (DFUs). This study aims to develop and use dressings based on chitosan derivatives for the sustained delivery of neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing. Three different derivatives, namely N-carboxymethyl chitosan, 5-methyl pyrrolidinone chitosan (MPC) and N-succinyl chitosan, are presented as potential biomaterials for wound healing applications. Our results show that MPC has the best fluid handling capacity and delivery profile, also being non-toxic to Raw 264.7 and HaCaT cells. NT-loaded and non-loaded MPC dressings were applied to control/diabetic wounds to evaluate their in vitro/in vivo performance. The results show that the former induced more rapid healing (50% wound area reduction) in the early phases of wound healing in diabetic mice. A NT-loaded MPC foam also reduced expression of the inflammatory cytokine TNF-α (P < 0.001) and decreased the amount of inflammatory infiltrate on day 3. On day 10 MMP-9 was reduced in diabetic skin (P < 0.001), significantly increasing fibroblast migration and collagen (COL1A1, COL1A2 and COL3A1) expression and deposition. These results suggest that MPC-based dressings may work as an effective support for sustained NT release to reduce DFUs.
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2013.09.040
Rights: openAccess
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
FCTUC Eng.Química - Artigos em Revistas Internacionais

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