Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27278
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dc.contributor.authorMoura, Liane I. F.-
dc.contributor.authorDias, Ana M. A.-
dc.contributor.authorLeal, Ermelindo C.-
dc.contributor.authorCarvalho, Lina-
dc.contributor.authorSousa, Hermínio C. de-
dc.contributor.authorCarvalho, Eugénia-
dc.date.accessioned2014-10-15T09:29:32Z-
dc.date.available2014-10-15T09:29:32Z-
dc.date.issued2014-02-
dc.identifier.citationMOURA, Liane I. F. [et. al] - Chitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healing. "Acta Biomaterialia". ISSN 1742-7061. Vol. 10 Nº. 2 (2014) p. 843-857por
dc.identifier.issn1742-7061-
dc.identifier.urihttp://hdl.handle.net/10316/27278-
dc.description.abstractOne important complication of diabetes mellitus is chronic, non-healing diabetic foot ulcers (DFUs). This study aims to develop and use dressings based on chitosan derivatives for the sustained delivery of neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing. Three different derivatives, namely N-carboxymethyl chitosan, 5-methyl pyrrolidinone chitosan (MPC) and N-succinyl chitosan, are presented as potential biomaterials for wound healing applications. Our results show that MPC has the best fluid handling capacity and delivery profile, also being non-toxic to Raw 264.7 and HaCaT cells. NT-loaded and non-loaded MPC dressings were applied to control/diabetic wounds to evaluate their in vitro/in vivo performance. The results show that the former induced more rapid healing (50% wound area reduction) in the early phases of wound healing in diabetic mice. A NT-loaded MPC foam also reduced expression of the inflammatory cytokine TNF-α (P < 0.001) and decreased the amount of inflammatory infiltrate on day 3. On day 10 MMP-9 was reduced in diabetic skin (P < 0.001), significantly increasing fibroblast migration and collagen (COL1A1, COL1A2 and COL3A1) expression and deposition. These results suggest that MPC-based dressings may work as an effective support for sustained NT release to reduce DFUs.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectChitosan derivativespor
dc.subjectWound dressingspor
dc.subjectDiabetic foot ulcerspor
dc.subjectNeurotensinpor
dc.subjectWound healingpor
dc.titleChitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healingpor
dc.typearticlepor
degois.publication.firstPage843por
degois.publication.lastPage857por
degois.publication.issue2por
degois.publication.titleActa Biomaterialiapor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1742706113005047por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.actbio.2013.09.040-
degois.publication.volume10por
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Medicina, Universidade de Coimbra-
crisitem.author.researchunitiNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG and ITQB)-
crisitem.author.orcid0000-0001-8349-4488-
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
FCTUC Eng.Química - Artigos em Revistas Internacionais
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