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Title: | Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells | Authors: | Vazão, Helena Neves, Ricardo Pires das Grãos, Mário Ferreira, Lino |
Issue Date: | 10-Mar-2011 | Publisher: | Public Library of Science | Citation: | VAZÃO, Helena - Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells. "PLoS ONE". ISSN 1932-6203. 6:3 (2011) e17771 | Serial title, monograph or event: | PLoS ONE | Volume: | 6 | Issue: | 3 | Abstract: | In this study we demonstrate that CD34+ cells derived from human embryonic stem cells (hESCs) have higher smooth muscle cell (SMC) potential than CD34− cells. We report that from all inductive signals tested, retinoic acid (RA) and platelet derived growth factor (PDGFBB) are the most effective agents in guiding the differentiation of CD34+ cells into smooth muscle progenitor cells (SMPCs) characterized by the expression of SMC genes and proteins, secretion of SMC-related cytokines, contraction in response to depolarization agents and vasoactive peptides and expression of SMC-related genes in a 3D environment. These cells are also characterized by a low organization of the contractile proteins and the contractility response is mediated by Ca2+, which involves the activation of Rho A/Rho kinase- and Ca2+/calmodulin (CaM)/myosin light chain kinase (MLCK)-dependent pathways. We further show that SMPCs obtained from the differentiation of CD34+ cells with RA, but not with PDGFBB, can be maturated in medium supplemented with endothelin-1 showing at the end individualized contractile filaments. Overall the hESC-derived SMCs presented in this work might be an unlimited source of SMCs for tissue engineering and regenerative medicine. | URI: | https://hdl.handle.net/10316/15680 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0017771 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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Towards the Maturation and Characterization of Smooth Muscle Cells.pdf | 1.34 MB | Adobe PDF | View/Open |
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