Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/15680
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dc.contributor.authorVazão, Helena-
dc.contributor.authorNeves, Ricardo Pires das-
dc.contributor.authorGrãos, Mário-
dc.contributor.authorFerreira, Lino-
dc.date.accessioned2011-08-12T15:28:57Z-
dc.date.available2011-08-12T15:28:57Z-
dc.date.issued2011-03-10-
dc.identifier.citationVAZÃO, Helena - Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells. "PLoS ONE". ISSN 1932-6203. 6:3 (2011) e17771-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10316/15680-
dc.description.abstractIn this study we demonstrate that CD34+ cells derived from human embryonic stem cells (hESCs) have higher smooth muscle cell (SMC) potential than CD34− cells. We report that from all inductive signals tested, retinoic acid (RA) and platelet derived growth factor (PDGFBB) are the most effective agents in guiding the differentiation of CD34+ cells into smooth muscle progenitor cells (SMPCs) characterized by the expression of SMC genes and proteins, secretion of SMC-related cytokines, contraction in response to depolarization agents and vasoactive peptides and expression of SMC-related genes in a 3D environment. These cells are also characterized by a low organization of the contractile proteins and the contractility response is mediated by Ca2+, which involves the activation of Rho A/Rho kinase- and Ca2+/calmodulin (CaM)/myosin light chain kinase (MLCK)-dependent pathways. We further show that SMPCs obtained from the differentiation of CD34+ cells with RA, but not with PDGFBB, can be maturated in medium supplemented with endothelin-1 showing at the end individualized contractile filaments. Overall the hESC-derived SMCs presented in this work might be an unlimited source of SMCs for tissue engineering and regenerative medicine.por
dc.description.sponsorshipMarie Curie-Reintegration Grantpor
dc.description.sponsorshipMIT-Portugal program-
dc.description.sponsorshipCrioestaminal-
dc.description.sponsorshipAssociação Viver a Ciência-
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (PTDC/SA-BEB/098468/2008 and PTDC/CTM/099659/2008 to L.F.; SFRH/BD/40077/2007 to H.V.)-
dc.language.isoengpor
dc.publisherPublic Library of Sciencepor
dc.rightsopenAccesspor
dc.titleTowards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cellspor
dc.typearticlepor
degois.publication.firstPagee17771por
degois.publication.issue3por
degois.publication.titlePLoS ONEpor
dc.peerreviewedYespor
dc.identifier.doi10.1371/journal.pone.0017771-
degois.publication.volume6por
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-5955-4804-
crisitem.author.orcid0000-0002-2707-1488-
crisitem.author.orcid0000-0001-8985-9302-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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