Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/115210
Title: | Multistep microreactions with proteins using electrocapture technology | Authors: | Astorga-Wells, Juan Bergman, Tomas Jörnvall, Hans |
Issue Date: | 1-May-2004 | Volume: | 76 | Issue: | 9 | Abstract: | A method to perform multistep reactions by means of electroimmobilization of a target molecule in a microflow stream is presented. A target protein is captured by the opposing effects between the hydrodynamic and electric forces, after which another medium is injected into the system. The second medium carries enzymes or other reagents, which are brought into contact with the target protein and react. The immobilization is reversed by disconnecting the electric field, upon which products are collected at the outlet of the device for analysis. On-line reduction, alkylation, and trypsin digestion of proteins is demonstrated and was monitored by MALDI mass spectrometry. | URI: | https://hdl.handle.net/10316/115210 | ISSN: | 0003-2700 | DOI: | 10.1021/ac0354342 | Rights: | embargoedAccess |
Appears in Collections: | FCDEF - Vários |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Guia para depósito no EG.pdf | 194.91 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
27
checked on Oct 28, 2024
WEB OF SCIENCETM
Citations
26
checked on Oct 2, 2024
Page view(s)
56
checked on Oct 23, 2024
Download(s)
47
checked on Oct 23, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.