Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/113583
Title: Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Induced under Heat Stress
Authors: Pereira, Cátia 
Castander-Olarieta, Ander
Montalbán, Itziar A.
Mendes, Vera M. 
Correia, Sandra 
Pedrosa, Ana 
Manadas, Bruno 
Moncaleán, Paloma
Canhoto, Jorge 
Keywords: Aleppo pine; conifers; metabolism; proteins; stress response
Issue Date: 13-Apr-2023
Publisher: MDPI
Project: CENTRO-08-5864-FSE-000031 
info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH/BD/123702/2016/PT 
UIDB/04539/2020 
info:eu-repo/grantAgreement/UIDP/04539/2020 
AGL2016-76143-C4-3R 
PINFRA/22125/2016 
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 24
Issue: 8
Abstract: Understanding the physiological and molecular adjustments occurring during tree stress response is of great importance for forest management and breeding programs. Somatic embryogenesis has been used as a model system to analyze various processes occurring during embryo development, including stress response mechanisms. In addition, "priming" plants with heat stress during somatic embryogenesis seems to favor the acquisition of plant resilience to extreme temperature conditions. In this sense, Pinus halepensis somatic embryogenesis was induced under different heat stress treatments (40 °C for 4 h, 50 °C for 30 min, and 60 °C for 5 min) and its effects on the proteome and the relative concentration of soluble sugars, sugar alcohols and amino acids of the embryonal masses obtained were assessed. Heat severely affected the production of proteins, and 27 proteins related to heat stress response were identified; the majority of the proteins with increased amounts in embryonal masses induced at higher temperatures consisted of enzymes involved in the regulation of metabolism (glycolysis, the tricarboxylic acid cycle, amino acid biosynthesis and flavonoids formation), DNA binding, cell division, transcription regulation and the life-cycle of proteins. Finally, significant differences in the concentrations of sucrose and amino acids, such as glutamine, glycine and cysteine, were found.
URI: https://hdl.handle.net/10316/113583
ISSN: 1422-0067
DOI: 10.3390/ijms24087211
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais

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