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Título: | Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria | Autor: | Inwezerua, Christiana Mendonça, Nuno Calhau, Vera Domingues, Sara Adeleke, Olufemi Ezekiel Silva, Gabriela Jorge da |
Palavras-chave: | Escherichia coli; extended-spectrum beta-lactamases; plasmid-mediated quinolone resistance; genetic mobile elements | Data: | 11-Jun-2014 | Editora: | Open Learning on Enteric Pathogens | Projeto: | C. Inwezerua was supported by a grant of the Scholarship Program for Young African Researchers of Coimbra Group, University of Coimbra, Portugal. N. Mendonça and S. Domingues were supported by the grants SFRH/BPD/45815/2008 and SFRH/BD/49061/2008, respectively, from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal. This work was supported financially by the ESCMID 2010 Research Grant from the European Society of Clinical Microbiology and Infectious Diseases, the PTDC/AGR-ALI/113953/2009 project from Fundação para a Ciência e a Tecnologia and by the Center for Pharmaceutical Studies, University of Coimbra. | Título da revista, periódico, livro ou evento: | Journal of Infection in Developing Countries | Volume: | 8 | Número: | 6 | Resumo: | Introduction: The main objective of the study was the molecular characterization of extended spectrum β-lactamases (ESBL) in Escherichia coli isolates collected from human and bovine samples in Oyo state, Nigeria. Methodology: Between August 2010-2011, 114 E. coli isolates were collected from hospitals (n = 57) and bovine (n = 57). PCR and sequencing were used for identification of ESBLs, upstream sequences, plasmid-mediated quinolone resistance (PMQR) genes and class 1 integrons. Plasmid incompatibility groups were identified among ESBL-positive isolates by PCR. Genetic relatdness was assessed by rep-PCR and MLST. Transfer of ESBL determinants to the recipient strain E. coli J53 was performed by broth mating assays. Results: CTX-M15 was the unique ESBL found in eight human isolates. Six CTX-M-15 producers also carry the aac(6’)-lb-cr gene and/or qnrB gene, and class 1 integrons. FIA, FIB, H11, H12, F, Y and K were the plasmid replicon types found. CTX-M-15 and PMQR determinants were transferred by conjugation in two E. coli assigned by MLST to ST131 and ST2695, a new allele. Conclusions: The study highlights the dissemination hability of CTX-M-15 associated with PMQR, and the presence of class 1 integrons, able to capture additional genes, justifying the urgent need of antimicrobial resistance surveillance in Nigeria. | URI: | https://hdl.handle.net/10316/109498 | ISSN: | 1972-2680 | DOI: | 10.3855/jidc.3430 | Direitos: | openAccess |
Aparece nas coleções: | FFUC- Artigos em Revistas Internacionais |
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Occurrence-of-extendedspectrum-betalactamases-in-human-and-bovine-isolates-of-Escherichia-coli-from-Oyo-state-NigeriaJournal-of-Infection-in-Developing-Countries.pdf | 552.08 kB | Adobe PDF | Ver/Abrir |
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