Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/109498
DC Field | Value | Language |
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dc.contributor.author | Inwezerua, Christiana | - |
dc.contributor.author | Mendonça, Nuno | - |
dc.contributor.author | Calhau, Vera | - |
dc.contributor.author | Domingues, Sara | - |
dc.contributor.author | Adeleke, Olufemi Ezekiel | - |
dc.contributor.author | Silva, Gabriela Jorge da | - |
dc.date.accessioned | 2023-10-18T08:12:33Z | - |
dc.date.available | 2023-10-18T08:12:33Z | - |
dc.date.issued | 2014-06-11 | - |
dc.identifier.issn | 1972-2680 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/109498 | - |
dc.description.abstract | Introduction: The main objective of the study was the molecular characterization of extended spectrum β-lactamases (ESBL) in Escherichia coli isolates collected from human and bovine samples in Oyo state, Nigeria. Methodology: Between August 2010-2011, 114 E. coli isolates were collected from hospitals (n = 57) and bovine (n = 57). PCR and sequencing were used for identification of ESBLs, upstream sequences, plasmid-mediated quinolone resistance (PMQR) genes and class 1 integrons. Plasmid incompatibility groups were identified among ESBL-positive isolates by PCR. Genetic relatdness was assessed by rep-PCR and MLST. Transfer of ESBL determinants to the recipient strain E. coli J53 was performed by broth mating assays. Results: CTX-M15 was the unique ESBL found in eight human isolates. Six CTX-M-15 producers also carry the aac(6’)-lb-cr gene and/or qnrB gene, and class 1 integrons. FIA, FIB, H11, H12, F, Y and K were the plasmid replicon types found. CTX-M-15 and PMQR determinants were transferred by conjugation in two E. coli assigned by MLST to ST131 and ST2695, a new allele. Conclusions: The study highlights the dissemination hability of CTX-M-15 associated with PMQR, and the presence of class 1 integrons, able to capture additional genes, justifying the urgent need of antimicrobial resistance surveillance in Nigeria. | pt |
dc.language.iso | eng | pt |
dc.publisher | Open Learning on Enteric Pathogens | pt |
dc.relation | C. Inwezerua was supported by a grant of the Scholarship Program for Young African Researchers of Coimbra Group, University of Coimbra, Portugal. N. Mendonça and S. Domingues were supported by the grants SFRH/BPD/45815/2008 and SFRH/BD/49061/2008, respectively, from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal. This work was supported financially by the ESCMID 2010 Research Grant from the European Society of Clinical Microbiology and Infectious Diseases, the PTDC/AGR-ALI/113953/2009 project from Fundação para a Ciência e a Tecnologia and by the Center for Pharmaceutical Studies, University of Coimbra. | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Escherichia coli | pt |
dc.subject | extended-spectrum beta-lactamases | pt |
dc.subject | plasmid-mediated quinolone resistance | pt |
dc.subject | genetic mobile elements | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Cattle | pt |
dc.subject.mesh | Developing Countries | pt |
dc.subject.mesh | Drug Resistance, Bacterial | pt |
dc.subject.mesh | Escherichia coli | pt |
dc.subject.mesh | Escherichia coli Infections | pt |
dc.subject.mesh | Escherichia coli Proteins | pt |
dc.subject.mesh | Genes, Bacterial | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Integrons | pt |
dc.subject.mesh | Microbial Sensitivity Tests | pt |
dc.subject.mesh | Nigeria | pt |
dc.subject.mesh | Plasmids | pt |
dc.subject.mesh | beta-Lactamases | pt |
dc.title | Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria | pt |
dc.type | article | - |
degois.publication.firstPage | 774 | pt |
degois.publication.lastPage | 779 | pt |
degois.publication.issue | 6 | pt |
degois.publication.title | Journal of Infection in Developing Countries | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3855/jidc.3430 | pt |
degois.publication.volume | 8 | pt |
dc.date.embargo | 2014-06-11 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | MARE - Marine and Environmental Sciences Centre | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-5567-282X | - |
crisitem.author.orcid | 0000-0002-8879-5113 | - |
crisitem.author.orcid | 0000-0002-7479-8540 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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Occurrence-of-extendedspectrum-betalactamases-in-human-and-bovine-isolates-of-Escherichia-coli-from-Oyo-state-NigeriaJournal-of-Infection-in-Developing-Countries.pdf | 552.08 kB | Adobe PDF | View/Open |
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