Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/108123
Title: Crosstalk between glial and glioblastoma cells triggers the "go-or-grow" phenotype of tumor cells
Authors: Oliveira, Ana Isabel
Anjo, Sandra I. 
Vieira de Castro, Joana
Serra, Sofia C.
Salgado, António J. 
Manadas, Bruno 
Costa, Bruno M.
Keywords: Glioblastoma; Glial cells; Tumor microenvironment; Secretome; Paracrine effect
Issue Date: 2-Oct-2017
Publisher: Springer Nature
Project: SFRH/BD/52287/2013 
SFRH/BD/81495/2011 
SFRH/BD/88121/2012 
PTDC/NEU-SCC/7051/2014 
PTDC/ NEU-NMC/0205/2012 
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013 
Serial title, monograph or event: Cell Communication and Signaling
Volume: 15
Issue: 1
Abstract: Background: Glioblastoma (GBM), the most malignant primary brain tumor, leads to poor and unpredictable clinical outcomes. Recent studies showed the tumor microenvironment has a critical role in regulating tumor growth by establishing a complex network of interactions with tumor cells. In this context, we investigated how GBM cells modulate resident glial cells, particularly their paracrine activity, and how this modulation can influence back on the malignant phenotype of GBM cells. Methods: Conditioned media (CM) of primary mouse glial cultures unexposed (unprimed) or exposed (primed) to the secretome of GL261 GBM cells were analyzed by proteomic analysis. Additionally, these CM were used in GBM cells to evaluate their impact in glioma cell viability, migration capacity and activation of tumor-related intracellular pathways. Results: The proteomic analysis revealed that the pre-exposure of glial cells to CM from GBM cells led to the upregulation of several proteins related to inflammatory response, cell adhesion and extracellular structure organization within the secretome of primed glial cells. At the functional levels, CM derived from unprimed glial cells favored an increase in GBM cell migration capacity, while CM from primed glial cells promoted cells viability. These effects on GBM cells were accompanied by activation of particular intracellular cancer-related pathways, mainly the MAPK/ERK pathway, which is a known regulator of cell proliferation. Conclusions: Together, our results suggest that glial cells can impact on the pathophysiology of GBM tumors, and that the secretome of GBM cells is able to modulate the secretome of neighboring glial cells, in a way that regulates the “go-or-grow” phenotypic switch of GBM cells.
URI: https://hdl.handle.net/10316/108123
ISSN: 1478-811X
DOI: 10.1186/s12964-017-0194-x
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

Show full item record

Page view(s)

94
checked on Oct 9, 2024

Download(s)

36
checked on Oct 9, 2024

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons