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https://hdl.handle.net/10316/107175
Title: | Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve | Authors: | Mendes, Vera M. Coelho, Margarida Tomé, Ângelo R. Cunha, Rodrigo A. Manadas, Bruno |
Keywords: | caffeine; LC-MS/MS; non-matched matrix calibration curve | Issue Date: | 7-Aug-2019 | Publisher: | MDPI | Project: | PTDC/NEU-NMC/0205/2012 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2019 SAICTPAC/0010/2015 PTDC/NEU-SCC/7051/2014 PTDC/BTM-TEC/29311/2017 PTDC/MEC-PSQ/30943/2017 PTDC/MED-NEU/27946/2017 |
Serial title, monograph or event: | Molecules | Volume: | 24 | Issue: | 16 | Abstract: | Caffeine is one of the most widely consumed psycho-stimulants. The study of the beneficial effects of caffeine consumption to decrease the risk of developing several neuropsychiatric pathologies is receiving increasing attention. Thus, accurate and sensitive methods have been developed, mainly by LC-MS/MS, in order to quantify caffeine and its metabolites. These quantifications of caffeine and its metabolites by LC-MS/MS require a considerable effort to select or find a surrogate matrix, without the compounds of interest, to be used in the calibration curves. Thus, we evaluated the possibility of using calibration curves prepared in solvent instead of calibration curves prepared in human plasma. Results show that the calibration curves prepared in solvent and in human plasma were similar by comparing their slopes and interceptions, and the accuracy and precision were within the limits of acceptance for both calibration curves. This work demonstrates that, by using internal standards, it is possible to use a calibration curve in solvent instead of a calibration curve in plasma to perform an accurate and precise quantification of caffeine and theobromine. | URI: | https://hdl.handle.net/10316/107175 | ISSN: | 1420-3049 | DOI: | 10.3390/molecules24162863 | Rights: | openAccess |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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