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Título: | Endophytic Lifestyle of Global Clones of Extended-Spectrum β-Lactamase-Producing Priority Pathogens in Fresh Vegetables: a Trojan Horse Strategy Favoring Human Colonization? | Autor: | Lopes, Ralf Fuentes-Castillo, Danny Fontana, Herrison Rodrigues, Larissa Dantas, Karine Cerdeira, Louise Henriques, Isabel Lincopan, Nilton |
Palavras-chave: | E. coli ST648; E. coli ST38; K. pneumoniae CC307; CTX-M-15; food; One Health; ESBL | Data: | 9-Fev-2021 | Editora: | American Society for Microbiology | Projeto: | Bill & Melinda Gates Foundation (Grand Challenges Explorations Brazil—new approaches to characterize the global burden of antimicrobial resistance, grant OPP1193112 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grants 2016/08593-9 and 15/21325-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Grants 443819/ 2018-1, 312249/2017-9 and 433128/2018-6) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, grant 1541491/2015-8, finance code 001) UIDP/50017/2020 UIDB/50017/ 2020 |
Título da revista, periódico, livro ou evento: | mSystems | Volume: | 6 | Número: | 1 | Resumo: | The global spread of antibiotic-resistant bacteria and their resistance genes is a critical issue that is no longer restricted to hospital settings, but also represents a growing problem involving environmental and food safety. In this study, we have performed a microbiological and genomic investigation of critical priority pathogens resistant to broad-spectrum cephalosporins and showing endophytic lifestyles in fresh vegetables sold in a country with high endemicity of extended-spectrum β-lactamases (ESBLs). We report the isolation of international high-risk clones of CTX-M-15-producing Escherichia coli, belonging to clonal complexes CC38 and CC648, and Klebsiella pneumoniae of complex CC307 from macerated tissue of surface-sterilized leaves of spinach, cabbage, arugula, and lettuce. Regardless of species, all ESBL-positive isolates were able to endophytically colonize common bean (Phaseolus vulgaris) seedlings, showed resistance to acid pH, and had a multidrug-resistant (MDR) profile to clinically relevant antibiotics (i.e., broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Genomic analysis of CTX-M-producing endophytic Enterobacterales revealed a wide resistome (antibiotics, biocides, disinfectants, and pesticides) and virulome, and genes for endophytic fitness and for withstanding acidic conditions. Transferable IncFIB and IncHI2A plasmids carried bla CTX-M-15 genes and, additionally, an IncFIB plasmid (named pKP301cro) also harbored genes encoding resistance to heavy metals. These data support the hypothesis that fresh vegetables marketed for consumption can act as a figurative Trojan horse for the hidden spread of international clones of critical WHO priority pathogens producing ESBLs, and/or their resistance genes, to humans and other animals, which is a critical issue within a food safety and broader public and environmental health perspective.IMPORTANCE Extended-spectrum β-lactamases (ESBL)-producing Enterobacterales are a leading cause of human and animal infections, being classified as critical priority pathogens by the World Health Organization. Epidemiological studies have shown that spread of ESBL-producing bacteria is not a problem restricted to hospitals, but also represents a growing problem involving environmental and food safety. In this regard, CTX-M-type β-lactamases have become the most widely distributed and clinically relevant ESBLs worldwide. Here, we have investigated the occurrence and genomic features of ESBL-producing Enterobacterales in surface-sterilized fresh vegetables. We have uncovered that international high-risk clones of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae harboring a wide resistome and virulome, carry additional genes for endophytic fitness and resistance to acidic conditions. Furthermore, we have demonstrated that these CTX-M-15-positive isolates are able to endophytically colonize plant tissues. Therefore, we believe that fresh vegetables can act as a figurative Trojan horse for the hidden spread of critical priority pathogens exhibiting endophytic lifestyles. | URI: | https://hdl.handle.net/10316/105277 | ISSN: | 2379-5077 | DOI: | 10.1128/mSystems.01125-20 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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Ficheiro | Descrição | Tamanho | Formato | |
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Endophytic Lifestyle of Global Clones of Extended-Spectrum.pdf | 5.73 MB | Adobe PDF | Ver/Abrir |
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