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Title: Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv
Authors: Santos, Joana
Cardoso, Miguel 
Moreira, Irina S. 
Gonçalves, João
Correia, João D. G.
Verde, Sandra Cabo
Melo, Rita 
Keywords: cell transfection; DNA plasmid; human epidermal growth factor receptor 2; molecular docking; recombinant protein
Issue Date: 29-Mar-2021
Publisher: MDPI AG
Project: FCT - 2020.08317.BD 
PTDC/QUINUC/ 30147/2017 
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 22
Issue: 7
Abstract: Biological therapies, such as recombinant proteins, are nowadays amongst the most promising approaches towards precision medicine. One of the most innovative methodologies currently available aimed at improving the production yield of recombinant proteins with minimization of costs relies on the combination of in silico studies to predict and deepen the understanding of the modified proteins with an experimental approach. The work described herein aims at the design and production of a biomimetic vector containing the single-chain variable domain fragment (scFv) of an anti-HER2 antibody fragment as a targeting motif fused with HIV gp41. Molecular modeling and docking studies were performed to develop the recombinant protein sequence. Subsequently, the DNA plasmid was produced and HEK-293T cells were transfected to evaluate the designed vector. The obtained results demonstrated that the plasmid construction is robust and can be expressed in the selected cell line. The multidisciplinary integrated in silico and experimental strategy adopted for the construction of a recombinant protein which can be used in HER2+-targeted therapy paves the way towards the production of other therapeutic proteins in a more cost-effective way.
ISSN: 1422-0067
DOI: 10.3390/ijms22073547
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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