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https://hdl.handle.net/10316/102616
Title: | Differential immuno-reactivity to genomic DNA, RNA and mitochondrial DNA is associated with auto-immunity | Authors: | Ivanova, Vilena V Khaiboullina, Svetlana F Cherenkova, Ekaterina E Martynova, Ekaterina V Nevzorova, Tatiana A Kunst, Michail A Sibgatullin, Timur B Maksudova, Adelia N Oliveira, Paulo J. Lombardi, Vincent C Palotas, Andras Rizvanov, Albert A |
Keywords: | Auto-antibody; Auto-antigens; Auto-immune disease; Biomarker; Mitochondri; Nucleic acid; Systemic lupus erythematosus (SLE) | Issue Date: | 2014 | Project: | Russian Government Program of Competitive Growth of Kazan Federal University and funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities. | Serial title, monograph or event: | Cellular Physiology and Biochemistry | Volume: | 34 | Issue: | 6 | Abstract: | Circulating auto-reactive antibodies are hallmark features of auto-immune diseases, however little is known with respect to the specificity of such bio-markers. In the present study, we investigated the specificity of anti-nucleic acid antibodies in the blood of subjects with systemic lupus erythematosus (SLE) and healthy controls. Methods: Sera from 12 SLE cases and 8 controls were evaluated for immuno-reactivity to purified RNA, DNA and mitochondrial DNA (mtDNA) by enzyme-linked immuno-sorbent assay (ELISA). Results: As expected, immuno-reactivity to total nucleic acids was significantly higher in subjects with SLE when compared to healthy controls, however a clear distinction was observed among the various nucleic acid sub-types, with sera from SLE subjects displaying the greatest immunoreactivity to RNA followed by mtDNA and then total DNA. Conclusion: The identification of auto-reactive antibodies can serve as highly sensitive biomarkers, although their specificity may not always allow diagnostic certainty. The knowledge that auto-antibodies in subjects with SLE display differential immuno-reactivity may help to improve existing diagnostics and may lead to a better understanding of the pathogenesis of auto-immune disorders. | URI: | https://hdl.handle.net/10316/102616 | ISSN: | 1421-9778 1015-8987 |
DOI: | 10.1159/000369663 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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