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Title: Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells
Authors: Ventura, Célia 
Marques, Catarina
Cadete, João
Vilar, Madalena
Pedrosa, Jorge F. S. 
Pinto, Fátima 
Fernandes, Susete Nogueira
da Rosa, Rafaela Raupp
Godinho, Maria Helena
Ferreira, Paulo J. T. 
Louro, Henriqueta
Silva, Maria João 
Keywords: nanofibrillated cellulose; nanocrystalline cellulose; nanotoxicology; cytotoxicity; genotoxicity; micronucleus assay
Issue Date: 2022
Publisher: MDPI
Project: info:eu-repo/grantAgreement/ ToxApp4NanoCELFI/| PTDC/SAU-PUB/32587/2017 
EUTOPIA CA17139/European Topology Interdisciplinary Action 
info:eu-repo/grantAgreement/POCI-01-0247-FEDER-021874/Inpactus Project—innovative products and technologies from eucalyptus 
Serial title, monograph or event: Journal of Xenobiotics
Volume: 12
Issue: 2
Abstract: Background: Nanocellulose is an innovative engineered nanomaterial with an enormous potential for use in a wide array of industrial and biomedical applications and with fast growing economic value. The expanding production of nanocellulose is leading to an increased human exposure, raising concerns about their potential health effects. This study was aimed at assessing the potential toxic and genotoxic effects of different nanocelluloses in two mammalian cell lines; (2) Methods: Two micro/nanocelluloses, produced with a TEMPO oxidation pre-treatment (CNFs) and an enzymatic pre-treatment (CMFs), and cellulose nanocrystals (CNCs) were tested in osteoblastic-like human cells (MG-63) and Chinese hamster lung fibroblasts (V79) using the MTT and clonogenic assays to analyse cytotoxicity, and the micronucleus assay to test genotoxicity; (3) Results: cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNCs, but not by the MTT assay; CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells; CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells; (4) Conclusions: All nanocelluloses revealed cytotoxicity and genotoxicity, although at different concentrations, that may be related to their physicochemical differences and availability for cell uptake, and to differences in the DNA damage response of the cell model.
ISSN: 2039-4713
DOI: 10.3390/jox12020009
Rights: openAccess
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais

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