Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/99908
DC FieldValueLanguage
dc.contributor.authorVentura, Célia-
dc.contributor.authorMarques, Catarina-
dc.contributor.authorCadete, João-
dc.contributor.authorVilar, Madalena-
dc.contributor.authorPedrosa, Jorge F. S.-
dc.contributor.authorPinto, Fátima-
dc.contributor.authorFernandes, Susete Nogueira-
dc.contributor.authorda Rosa, Rafaela Raupp-
dc.contributor.authorGodinho, Maria Helena-
dc.contributor.authorFerreira, Paulo J. T.-
dc.contributor.authorLouro, Henriqueta-
dc.contributor.authorSilva, Maria João-
dc.date.accessioned2022-04-21T13:56:50Z-
dc.date.available2022-04-21T13:56:50Z-
dc.date.issued2022-
dc.identifier.issn2039-4713pt
dc.identifier.urihttps://hdl.handle.net/10316/99908-
dc.description.abstractBackground: Nanocellulose is an innovative engineered nanomaterial with an enormous potential for use in a wide array of industrial and biomedical applications and with fast growing economic value. The expanding production of nanocellulose is leading to an increased human exposure, raising concerns about their potential health effects. This study was aimed at assessing the potential toxic and genotoxic effects of different nanocelluloses in two mammalian cell lines; (2) Methods: Two micro/nanocelluloses, produced with a TEMPO oxidation pre-treatment (CNFs) and an enzymatic pre-treatment (CMFs), and cellulose nanocrystals (CNCs) were tested in osteoblastic-like human cells (MG-63) and Chinese hamster lung fibroblasts (V79) using the MTT and clonogenic assays to analyse cytotoxicity, and the micronucleus assay to test genotoxicity; (3) Results: cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNCs, but not by the MTT assay; CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells; CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells; (4) Conclusions: All nanocelluloses revealed cytotoxicity and genotoxicity, although at different concentrations, that may be related to their physicochemical differences and availability for cell uptake, and to differences in the DNA damage response of the cell model.pt
dc.description.sponsorshipThe research was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The following funding is also acknowledged: POCI-01-0145-FEDER-007688 (UIDB/50025/2020-2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); ToxOmics (UIDP/00009/2020; UIDB/00009/2020); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—innovative products and technologies from eucalyptus (Project N.◦ 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 n.º 246/AXIS II/2017).pt
dc.language.isoengpt
dc.publisherMDPIpt
dc.relationinfo:eu-repo/grantAgreement/ ToxApp4NanoCELFI/| PTDC/SAU-PUB/32587/2017pt
dc.relationUIDB/50025/2020-2023pt
dc.relationinfo:eu-repo/grantAgreement/POCI‐01‐0145‐FEDER‐007688pt
dc.relationinfo:eu-repo/grantAgreement/NanoCell2SEC/PTDC/CTM-REF/30529/2017pt
dc.relationCIEPQPF/UIDB/00102/2020pt
dc.relationEUTOPIA CA17139/European Topology Interdisciplinary Actionpt
dc.relationinfo:eu-repo/grantAgreement/POCI-01-0247-FEDER-021874/Inpactus Project—innovative products and technologies from eucalyptuspt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectnanofibrillated cellulosept
dc.subjectnanocrystalline cellulosept
dc.subjectnanotoxicologypt
dc.subjectcytotoxicitypt
dc.subjectgenotoxicitypt
dc.subjectmicronucleus assaypt
dc.titleGenotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cellspt
dc.typearticle-
degois.publication.firstPage91pt
degois.publication.lastPage108pt
degois.publication.issue2pt
degois.publication.titleJournal of Xenobioticspt
dc.peerreviewedyespt
dc.identifier.doi10.3390/jox12020009pt
degois.publication.volume12pt
dc.date.embargo2022-01-01*
uc.date.periodoEmbargo0pt
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.researchunitCIEPQPF – Chemical Process Engineering and Forest Products Research Centre-
crisitem.author.researchunitCIEPQPF – Chemical Process Engineering and Forest Products Research Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-6900-1592-
crisitem.author.orcid0000-0002-4503-6811-
crisitem.author.orcid0000-0002-6060-0716-
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
jox-12-00009.pdf5.68 MBAdobe PDFView/Open
Show simple item record

Page view(s)

115
checked on Apr 10, 2024

Download(s)

39
checked on Apr 10, 2024

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons