Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/96936
Title: Quantitative analysis of neuronal mitochondrial movement reveals patterns resulting from neurotoxicity of rotenone and 6-hydroxydopamine
Authors: Simões, Rui F. 
Pino, Rute
Soares, Maurício Moreira 
Kovarova, Jaromira 
Neuzil, Jiri 
Travasso, Rui 
Oliveira, Paulo J.
Cunha-Oliveira, Teresa 
Pereira, Francisco B.
Keywords: Exploratory data analysis; Live cell imaging; Mitochondria movement; Neurotoxicants; Principal component analysis; Trajectory descriptors
Issue Date: 2021
Publisher: Wiley
Serial title, monograph or event: FASEB Journal
Volume: 35
Issue: 12
Abstract: Alterations in mitochondrial dynamics, including their intracellular trafficking, are common early manifestations of neuronal degeneration. However, current methodologies used to study mitochondrial trafficking events rely on parameters that are primarily altered in later stages of neurodegeneration. Our objective was to establish a reliable applied statistical analysis to detect early alterations in neuronal mitochondrial trafficking. We propose a novel quantitative analysis of mitochondria trajectories based on innovative movement descriptors, including straightness, efficiency, anisotropy, and kurtosis. We evaluated time- and dose-dependent alterations in trajectory descriptors using biological data from differentiated SH-SY5Y cells treated with the mitochondrial toxicants 6-hydroxydopamine and rotenone. MitoTracker Red CMXRos-labelled mitochondria movement was analyzed by total internal reflection fluorescence microscopy followed by computational modelling to describe the process. Based on the aforementioned trajectory descriptors, this innovative analysis of mitochondria trajectories provides insights into mitochondrial movement characteristics and can be a consistent and sensitive method to detect alterations in mitochondrial trafficking occurring in the earliest time points of neurodegeneration.
URI: http://hdl.handle.net/10316/96936
ISSN: 0892-6638
1530-6860
DOI: 10.1096/fj.202100899R
Rights: openAccess
Appears in Collections:I&D CFis - Artigos em Revistas Internacionais
I&D CISUC - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Neuzil522434-Accepted.pdf1.35 MBAdobe PDFView/Open
Show full item record

WEB OF SCIENCETM
Citations

1
checked on Aug 2, 2022

Page view(s)

54
checked on Aug 5, 2022

Download(s)

45
checked on Aug 5, 2022

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons