Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/96936
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dc.contributor.authorSimões, Rui F.-
dc.contributor.authorPino, Rute-
dc.contributor.authorSoares, Maurício Moreira-
dc.contributor.authorKovarova, Jaromira-
dc.contributor.authorNeuzil, Jiri-
dc.contributor.authorTravasso, Rui-
dc.contributor.authorOliveira, Paulo J.-
dc.contributor.authorCunha-Oliveira, Teresa-
dc.contributor.authorPereira, Francisco B.-
dc.date.accessioned2022-01-07T16:54:07Z-
dc.date.available2022-01-07T16:54:07Z-
dc.date.issued2021-
dc.identifier.issn0892-6638pt
dc.identifier.issn1530-6860pt
dc.identifier.urihttp://hdl.handle.net/10316/96936-
dc.description.abstractAlterations in mitochondrial dynamics, including their intracellular trafficking, are common early manifestations of neuronal degeneration. However, current methodologies used to study mitochondrial trafficking events rely on parameters that are primarily altered in later stages of neurodegeneration. Our objective was to establish a reliable applied statistical analysis to detect early alterations in neuronal mitochondrial trafficking. We propose a novel quantitative analysis of mitochondria trajectories based on innovative movement descriptors, including straightness, efficiency, anisotropy, and kurtosis. We evaluated time- and dose-dependent alterations in trajectory descriptors using biological data from differentiated SH-SY5Y cells treated with the mitochondrial toxicants 6-hydroxydopamine and rotenone. MitoTracker Red CMXRos-labelled mitochondria movement was analyzed by total internal reflection fluorescence microscopy followed by computational modelling to describe the process. Based on the aforementioned trajectory descriptors, this innovative analysis of mitochondria trajectories provides insights into mitochondrial movement characteristics and can be a consistent and sensitive method to detect alterations in mitochondrial trafficking occurring in the earliest time points of neurodegeneration.pt
dc.language.isoengpt
dc.publisherWileypt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt
dc.subjectExploratory data analysispt
dc.subjectLive cell imagingpt
dc.subjectMitochondria movementpt
dc.subjectNeurotoxicantspt
dc.subjectPrincipal component analysispt
dc.subjectTrajectory descriptorspt
dc.subject.meshAdrenergic Agentspt
dc.subject.meshCell Differentiationpt
dc.subject.meshHumanspt
dc.subject.meshMitochondriapt
dc.subject.meshNeuroblastomapt
dc.subject.meshNeuronspt
dc.subject.meshOxidopaminept
dc.subject.meshRotenonept
dc.subject.meshUncoupling Agentspt
dc.subject.meshMitochondrial Dynamicspt
dc.titleQuantitative analysis of neuronal mitochondrial movement reveals patterns resulting from neurotoxicity of rotenone and 6-hydroxydopaminept
dc.typearticle-
degois.publication.firstPagee22024pt
degois.publication.issue12pt
degois.publication.titleFASEB Journalpt
dc.peerreviewedyespt
dc.identifier.doi10.1096/fj.202100899Rpt
degois.publication.volume35pt
dc.date.embargo2021-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-5982-8983-
crisitem.author.orcid0000-0001-9928-7256-
crisitem.author.orcid0000-0001-6078-0721-
crisitem.author.orcid0000-0002-7382-0339-
Appears in Collections:I&D CFis - Artigos em Revistas Internacionais
I&D CISUC - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
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This item is licensed under a Creative Commons License Creative Commons