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Title: Electrochemical immunosensor for detection of CA 15-3 biomarker in point-of-care
Authors: Rebelo, Tânia S.C.R.
Ribeiro, José A.
Sales, M. Goreti F. 
Pereira, Carlos M.
Keywords: CA 15-3; Cancer biomarker; Electrochemical biosensor; Immunosensor; Screen-printed electrode (SPE); Point-of-care (PoC) analysis
Issue Date: 2021
Publisher: Elsevier
Project: info:eu-repo/grantAgreement/FCT/PEst-C/QUI/UIDB/0081/2020/PT 
NORTE-01-0247-FEDER-017834 (project RamSERS) 
Serial title, monograph or event: Sensing and Bio-Sensing Research
Volume: 33
Abstract: This work reports the development of a simple and rapid electrochemical immunosensor for the determination of breast cancer biomarker Cancer Antigen 15–3 (CA 15–3). Disposable and cost-effective chips, consisting of gold screen-printed electrodes (AuSPEs), were used to develop the portable electrochemical devices for monitoring the biomarker in point-of-care (PoC), under clinical context. The biosensor preparation consisted of two simple steps. First, a self-assembled monolayer (SAM) of mer-captosuccinic acid (MSA) was formed at the AuSPE surface. Then, the CA 15–3 antibody was covalently bound to the carboxylic groups standing at the electrode surface using EDC/NHS chemistry. The performance of the developed immunosensor was evaluated by assessing the sensor sensitivity, linear response interval, selectivity and detection limit (LOD). The developed immunosensor provided a wide linear concentration range (from 1.0 to 1000 U mL
ISSN: 22141804
DOI: 10.1016/j.sbsr.2021.100445
Rights: openAccess
Appears in Collections:I&D CQC - Artigos em Revistas Internacionais

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