Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/95606
DC FieldValueLanguage
dc.contributor.authorRebelo, Tânia S.C.R.-
dc.contributor.authorRibeiro, José A.-
dc.contributor.authorSales, M. Goreti F.-
dc.contributor.authorPereira, Carlos M.-
dc.date.accessioned2021-08-17T15:15:14Z-
dc.date.available2021-08-17T15:15:14Z-
dc.date.issued2021-
dc.identifier.issn22141804pt
dc.identifier.urihttp://hdl.handle.net/10316/95606-
dc.description.abstractThis work reports the development of a simple and rapid electrochemical immunosensor for the determination of breast cancer biomarker Cancer Antigen 15–3 (CA 15–3). Disposable and cost-effective chips, consisting of gold screen-printed electrodes (AuSPEs), were used to develop the portable electrochemical devices for monitoring the biomarker in point-of-care (PoC), under clinical context. The biosensor preparation consisted of two simple steps. First, a self-assembled monolayer (SAM) of mer-captosuccinic acid (MSA) was formed at the AuSPE surface. Then, the CA 15–3 antibody was covalently bound to the carboxylic groups standing at the electrode surface using EDC/NHS chemistry. The performance of the developed immunosensor was evaluated by assessing the sensor sensitivity, linear response interval, selectivity and detection limit (LOD). The developed immunosensor provided a wide linear concentration range (from 1.0 to 1000 U mLpt
dc.language.isoengpt
dc.publisherElsevierpt
dc.relationinfo:eu-repo/grantAgreement/FCT/PEst-C/QUI/UIDB/0081/2020/PTpt
dc.relationNORTE-01-0247-FEDER-017834 (project RamSERS)pt
dc.relationSFRH/BPD/105395/2014pt
dc.relationSFRH/BSAB/150320/2019pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt
dc.subjectCA 15-3pt
dc.subjectCancer biomarkerpt
dc.subjectElectrochemical biosensorpt
dc.subjectImmunosensorpt
dc.subjectScreen-printed electrode (SPE)pt
dc.subjectPoint-of-care (PoC) analysispt
dc.titleElectrochemical immunosensor for detection of CA 15-3 biomarker in point-of-carept
dc.typearticle-
degois.publication.firstPage100445pt
degois.publication.titleSensing and Bio-Sensing Researchpt
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2214180421000507pt
dc.peerreviewedyespt
dc.identifier.doi10.1016/j.sbsr.2021.100445pt
degois.publication.volume33pt
dc.date.embargo2021-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.orcid0000-0001-9936-7336-
Appears in Collections:I&D CQC - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
1-s2.0-S2214180421000507-main.pdf1.38 MBAdobe PDFView/Open
Show simple item record

Page view(s)

39
checked on Oct 8, 2021

Download(s)

16
checked on Oct 8, 2021

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons