Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/95606
DC Field | Value | Language |
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dc.contributor.author | Rebelo, Tânia S.C.R. | - |
dc.contributor.author | Ribeiro, José A. | - |
dc.contributor.author | Sales, M. Goreti F. | - |
dc.contributor.author | Pereira, Carlos M. | - |
dc.date.accessioned | 2021-08-17T15:15:14Z | - |
dc.date.available | 2021-08-17T15:15:14Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 22141804 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/95606 | - |
dc.description.abstract | This work reports the development of a simple and rapid electrochemical immunosensor for the determination of breast cancer biomarker Cancer Antigen 15–3 (CA 15–3). Disposable and cost-effective chips, consisting of gold screen-printed electrodes (AuSPEs), were used to develop the portable electrochemical devices for monitoring the biomarker in point-of-care (PoC), under clinical context. The biosensor preparation consisted of two simple steps. First, a self-assembled monolayer (SAM) of mer-captosuccinic acid (MSA) was formed at the AuSPE surface. Then, the CA 15–3 antibody was covalently bound to the carboxylic groups standing at the electrode surface using EDC/NHS chemistry. The performance of the developed immunosensor was evaluated by assessing the sensor sensitivity, linear response interval, selectivity and detection limit (LOD). The developed immunosensor provided a wide linear concentration range (from 1.0 to 1000 U mL | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/PEst-C/QUI/UIDB/0081/2020/PT | pt |
dc.relation | NORTE-01-0247-FEDER-017834 (project RamSERS) | pt |
dc.relation | SFRH/BPD/105395/2014 | pt |
dc.relation | SFRH/BSAB/150320/2019 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt |
dc.subject | CA 15-3 | pt |
dc.subject | Cancer biomarker | pt |
dc.subject | Electrochemical biosensor | pt |
dc.subject | Immunosensor | pt |
dc.subject | Screen-printed electrode (SPE) | pt |
dc.subject | Point-of-care (PoC) analysis | pt |
dc.title | Electrochemical immunosensor for detection of CA 15-3 biomarker in point-of-care | pt |
dc.type | article | - |
degois.publication.firstPage | 100445 | pt |
degois.publication.title | Sensing and Bio-Sensing Research | pt |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2214180421000507 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1016/j.sbsr.2021.100445 | pt |
degois.publication.volume | 33 | pt |
dc.date.embargo | 2021-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
crisitem.author.orcid | 0000-0001-9936-7336 | - |
Appears in Collections: | I&D CQC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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1-s2.0-S2214180421000507-main.pdf | 1.38 MB | Adobe PDF | View/Open |
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