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Title: Characterization of ATP release from cultures enriched in cholinergic amacrine-like neurons
Authors: Santos, Paulo F. 
Caramelo, Olga L. 
Carvalho, Arsélio P. 
Duarte, Carlos B. 
Issue Date: 1999
Citation: Journal of Neurobiology. 41:3 (1999) 340-348
Abstract: Adenosine triphosphate (ATP) has been proposed to play a role as a neurotransmitter in the retina, but not much attention has been given to the regulation of ATP release from retinal neurons. In this work, we investigated the release of ATP from cultures enriched in amacrine-like neurons. Depolarization of the cells with KCl, or activation of alpha-amino-3-hydroxy- 5-methyl-4-isoxazole-propionate (AMPA) receptors, evoked the release of ATP, as determined by the luciferin/luciferase luminescent method. The ATP release was found to be largely Ca2+ dependent and sensitive to the botulinum neurotoxin A, which indicates that the ATP released by cultured retinal neurons originated from an exocytotic pool. Nitrendipine and omega-Agatoxin IVA, but not by omega-Conotoxin GVIA, partially blocked the release of ATP, indicating that in these cells, the Ca2+ influx necessary to trigger the release of ATP occurs in part through the L- and the P/Q types of voltage-sensitive Ca2+ channels (VSCC), but not through N-type VSCC. The release of ATP increased in the presence of adenosine deaminase, or in the presence of 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), an adenosine A1 receptor antagonist, showing that the release is tonically inhibited by the adenosine A1 receptors. To our knowledge, this is the first report showing the release of endogenous ATP from a retinal preparation. © 1999 John Wiley & Sons, Inc. J Neurobiol 41: 340-348, 1999
DOI: 10.1002/(SICI)1097-4695(19991115)41:3<340::AID-NEU3>3.0.CO;2-8
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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