Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8148
DC FieldValueLanguage
dc.contributor.authorVicente, Joaquim A. F.-
dc.contributor.authorPeixoto, Francisco-
dc.contributor.authorLopes, M. Ludovina-
dc.contributor.authorMadeira, Vítor M. C.-
dc.date.accessioned2009-02-09T11:13:31Z-
dc.date.available2009-02-09T11:13:31Z-
dc.date.issued2001en_US
dc.identifier.citationJournal of Biochemical and Molecular Toxicology. 15:6 (2001) 322-330en_US
dc.identifier.urihttp://hdl.handle.net/10316/8148-
dc.description.abstractParaquat herbicide is toxic to animals, including humans, via putative toxicity mechanisms associated to microsomal and mitochondrial redox systems. It is also believed to act in plants by generating highly reactive oxygen free radicals from electrons of photosystem I on exposure to light. Paraquat also acts on non-chlorophyllous plant tissues, where mitochondria are candidate targets, as in animal tissues. Therefore, we compared the interaction of paraquat with the mitochondrial bioenergetics of potato tuber, using rat liver mitochondria as a reference. Paraquat depressed succinate-dependent mitochondrial Deltapsi, with simultaneous stimulation of state 4 O2 consumption. It also induced a slow time-dependent effect for respiration of succinate, exogenous NADH, and N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD)/ascorbate, which was more pronounced in rat than in potato mitochondria. However, with potato tuber mitochondria, the Deltapsi promoted by complex-I-dependent respiration is insensitive to this effect, indicating a protection against paraquat radical afforded by complex I redox activity, which was just the reverse of to the findings for rat liver mitochondria. The experimental set up with the tetraphenyl phosphonium (TPP+)-electrode also indivated production of the paraquat radical in mitochondria, also suggesting its accessibility to the outside space. The different activities of protective antioxidant agents can contribute to explain the different sensitivities of both kinds of mitochondria. Values of SOD activity and alpha-tocopherol detected in potato mitochondria were significantly higher than in rat mitochondria, which, in turn, revealed higher values of lipid peroxidation induced by paraquat. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:322-330, 2001en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleDifferential sensitivities of plant and animal mitochondria to the herbicide paraquaten_US
dc.typearticleen_US
dc.identifier.doi10.1002/jbt.10010en_US
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
obra.pdf215.82 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

37
checked on May 29, 2020

WEB OF SCIENCETM
Citations 5

30
checked on Oct 22, 2020

Page view(s)

162
checked on Oct 27, 2020

Download(s)

106
checked on Oct 27, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.