Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8127
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dc.contributor.authorRodrigues, Tiago B.-
dc.contributor.authorGray, Heather L.-
dc.contributor.authorBenito, Marina-
dc.contributor.authorGarrido, Susana-
dc.contributor.authorSierra, Alejandra-
dc.contributor.authorGeraldes, Carlos F.-
dc.contributor.authorBallesteros, Paloma-
dc.contributor.authorCerdán, Sebastián-
dc.date.accessioned2009-02-09T11:10:31Z-
dc.date.available2009-02-09T11:10:31Z-
dc.date.issued2005en_US
dc.identifier.citationJournal of Neuroscience Research. 79:1-2 (2005) 119-127en_US
dc.identifier.urihttp://hdl.handle.net/10316/8127-
dc.description.abstractWe report a novel (13C, 2H) nuclear magnetic resonance (NMR) procedure to investigate lactate recycling through the monocarboxylate transporter of the plasma membrane of cells in culture. C6 glioma cells were incubated with [3-13C]lactate in Krebs-Henseleit Buffer containing 50% 2H2O (vol/vol) for up to 30 hr. 13C NMR analysis of aliquots progressively taken from the medium, showed: (1) a linearly decreasing singlet at sim20.85 parts per million (ppm; -0.119 mumol/mg protein/hr) derived from the methyl carbon of [3-13C]lactate; and (2) an exponentially increasing shifted singlet at sim20.74 ppm (0.227 mumol/ mg protein/hr) from the methyl carbon of [3-13C, 2-2H]lactate. The shifted singlet appears because during its transit through the cytosol, [3-13C]lactate generates [3-13C, 2-2H]lactate in the lactate dehydrogenase (LDH) equilibrium, which may return to the incubation medium through the reversible monocarboxylate carrier. The methyl group of [3-13C, 2-2H]lactate is shifted -0.11 ppm with respect to that of [3-13C]lactate, making it possible to distinguish between both molecules by 13C NMR. During incubations with 2.5 mM [1-13C]glucose and 3.98 mM [U-13C3]lactate or with 2.5 mM [1-13C]glucose and 3.93 mM [2-13C]pyruvate, C2-deuterated lactate was produced only from [1-13C]glucose or [U-13C3]lactate, revealing that this deuteration process is redox sensitive. When [1-13C]glucose and [U-13C3]lactate were used as substrates, no significant [3-13C]lactate production from [1-13C]glucose was detected, suggesting that glycolytic lactate production may be stopped under the high lactate concentrations prevailing under mild hypoxic or ischemic episodes or during cerebral activation. © 2004 Wiley-Liss, Inc.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleFutile cycling of lactate through the plasma membrane of C6 glioma cells as detected by (13C, 2H) NMRen_US
dc.typearticleen_US
dc.identifier.doi10.1002/jnr.20308en_US
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Economics-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCeBER – Centre for Business and Economics Research-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0001-5229-3130-
crisitem.author.orcid0000-0002-0837-8329-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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