Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8097
DC Field | Value | Language |
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dc.contributor.author | Montezinho, L. P. | - |
dc.contributor.author | Duarte, C. B. | - |
dc.contributor.author | Fonseca, C. P. | - |
dc.contributor.author | Glinka, Y. | - |
dc.contributor.author | Layden, B. | - |
dc.contributor.author | Freitas, D. Mota de | - |
dc.contributor.author | Geraldes, C. F. G. C. | - |
dc.contributor.author | Castro, M. M. C. A. | - |
dc.date.accessioned | 2009-02-09T11:10:55Z | - |
dc.date.available | 2009-02-09T11:10:55Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Journal of Neurochemistry. 90:4 (2004) 920-930 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8097 | - |
dc.description.abstract | In this work, we studied the effect of intracellular 3',5'-cyclic adenosine monophosphate (cAMP) on Li+ transport in SH-SY5Y cells. The cells were stimulated with forskolin, an adenylate cyclase activator, or with the cAMP analogue, dibutyryl-cAMP. It was observed that under forskolin stimulation both the Li+ influx rate constant and the Li+ accumulation in these cells were increased. Dibutyryl-cAMP also increased Li+ uptake and identical results were obtained with cortical and hippocampal neurons. The inhibitor of the Na+/Ca2+ exchanger, KB-R7943, reduced the influx of Li+ under resting conditions, and completely inhibited the effect of forskolin on the accumulation of the cation. Intracellular Ca2+ chelation, or inhibition of N-type voltage-sensitive Ca2+ channels, or inhibition of cAMP-dependent protein kinase (PKA) also abolished the effect of forskolin on Li+ uptake. The involvement of Ca2+ on forskolin-induced Li+ uptake was confirmed by intracellular free Ca2+ measurements using fluorescence spectroscopy. Exposure of SH-SY5Y cells to 1 mm Li+ for 24 h increased basal cAMP levels, but preincubation with Li+, at the same concentration, decreased cAMP production in response to forskolin. To summarize, these results demonstrate that intracellular cAMP levels regulate the uptake of Li+ in a Ca2+-dependent manner, and indicate that Li+ plays an important role in the homeostasis of this second messenger in neuronal cells. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Intracellular lithium and cyclic AMP levels are mutually regulated in neuronal cells | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1111/j.1471-4159.2004.02551.x | en_US |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-1474-0208 | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
crisitem.author.orcid | 0000-0001-6811-3878 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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