Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/7832
DC Field | Value | Language |
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dc.contributor.author | Egorova, K. | - |
dc.contributor.author | Grudieva, T. | - |
dc.contributor.author | Morinez, C. | - |
dc.contributor.author | Kube, J. | - |
dc.contributor.author | Santos, H. | - |
dc.contributor.author | Costa, M. da | - |
dc.contributor.author | Antranikian, G. | - |
dc.date.accessioned | 2009-02-17T10:35:10Z | - |
dc.date.available | 2009-02-17T10:35:10Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Applied Microbiology and Biotechnology. 75:5 (2007) 1039-1045 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/7832 | - |
dc.description.abstract | Abstract A production process, using upshock fermentation and osmotic downshock, for the effective production/excretion of mannosylglycerate (MG) by the trehalose-deficient mutant of the strain Thermus thermophilus RQ-1 has been developed. In the first phase of fed-batch fermentation, the knockout mutant was grown at 70°C on a NaCl-free medium. After the culture reached the end of the exponential growth phase, upshift in temperature and NaCl concentration was applied. The temperature was increased to 77°C, and NaCl was added up to 3.0% and kept constant during the second phase of fermentation. Although this shift in cultivation parameters caused a dramatic drop of cell density, a significant improvement in accumulation of MG up to 0.64 µmol/mg protein compared to batch fermentations (0.31 µmol/mg protein) was achieved. A total yield of 4.6 g MG/l of fermentation broth was obtained in the dialysis bioreactor with a productivity of 0.29 g MG l-1 h-1. The solute was released from the harvested biomass by osmotic downshock using demineralized water at 70°C. More than 90% of the intracellularly accumulated solute was recovered from the water fraction. The process was very efficient, as hyperosmotic shock, release of the solute, and reiterative fed-batch fermentation could be repeated at least four times. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | High yield of mannosylglycerate production by upshock fermentation and bacterial milking of trehalose-deficient mutant Thermus thermophilus RQ-1 | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1007/s00253-007-0915-y | en_US |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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