Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/7580
DC FieldValueLanguage
dc.contributor.authorMonteiro, João-
dc.contributor.authorVideira, Romeu-
dc.contributor.authorMatos, Manuel-
dc.contributor.authorDinis, Augusto-
dc.contributor.authorJurado, Amália-
dc.date.accessioned2009-02-17T10:19:08Z-
dc.date.available2009-02-17T10:19:08Z-
dc.date.issued2008en_US
dc.identifier.citationApplied Biochemistry and Biotechnology. 150:3 (2008) 243-257en_US
dc.identifier.urihttps://hdl.handle.net/10316/7580-
dc.description.abstractAbstract The Gram-positive bacterium, Bacillus stearothermophilus, was used as a model organism to identify the non-selective toxic effects of the currently used insecticide methoprene (isopropyl(2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate). A significant decrease of the yield of bacterial cultures and a premature appearance of ultrastructural abnormalities in cells cultured in the presence of the insecticide were taken as indicators of cytotoxicity. A putative correlation of this cytotoxicity with methoprene-induced perturbations on membrane lipid organization was investigated, using differential scanning calorimetry and the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its propionic acid derivative (DPH–PA). The membrane physical effects depended on the lipid bilayer composition and packing. The most striking effect was a progressive broadening and shifting to lower temperatures, with increasing methoprene concentrations, of the main transition phase of the dimyristoyl- or dipalmitoylphosphatidylcholine bilayers and of the lateral phase separation of liposomes reconstituted with the lipid extracts of B. stearothermophilus.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleNon-Selective Toxicological Effects of the Insect Juvenile Hormone Analogue Methoprene. A Membrane Biophysical Approachen_US
dc.typearticleen_US
dc.identifier.doi10.1007/s12010-007-8127-6en_US
uc.controloAutoridadeSim-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.cerifentitytypePublications-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-4170-0092-
crisitem.author.orcid0000-0001-7095-5337-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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