Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5366
Title: Toxicity of methoprene as assessed by the use of a model microorganism
Authors: Monteiro, J. P. 
Jurado, A. S. 
Moreno, A. J. M. 
Madeira, V. M. C. 
Keywords: Methoprene; Bacillus stearothermophilus; Bacterial growth; Lipid membrane; Respiratory activity
Issue Date: 2005
Citation: Toxicology in Vitro. 19:7 (2005) 951-956
Abstract: Methoprene is an insect juvenile growth hormone mimic, commonly used as a pesticide. Although widely used for the control of several pests, toxic effects on organisms of different phyla have been reported. These events triggered studies to clarify the mechanisms of toxicity of this insecticide putatively involved in ecological issues. Here we show the effect of methoprene on the normal cell growth and viability of a strain of the thermophilic eubacterium Bacillus stearothermophilus, previously used as a model for toxicological evaluation of other environment pollutants. Respiration studies were also carried out attempting to identify a putative target for the cytotoxic action of methoprene. Cell growth was affected and a decrease of the number of viable cells was observed as a result of the addition of methoprene to the growth medium, an effect reverted by the presence of Ca2+. Methoprene also inhibited the redox flow of B. stearothermophilus protoplasts before the cytochrome oxidase segment, an effect further studied by individually assessing the enzymatic activities of the respiratory complexes. This study suggests that methoprene membrane interaction and perturbation of cell bioenergetics may underlie the mechanism of toxicity of this compound in non-target organisms.
URI: http://hdl.handle.net/10316/5366
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat 
file9764969aa14a4a0e8c90e6d51b83d770.pdf146.66 kBAdobe PDFView/Open
Show full item record

Page view(s) 20

488
checked on Jun 11, 2019

Download(s) 50

181
checked on Jun 11, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.