Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5358
DC Field | Value | Language |
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dc.contributor.author | Saavedra, Ana | - |
dc.contributor.author | Baltazar, Graça | - |
dc.contributor.author | Carvalho, Caetana M. | - |
dc.contributor.author | Duarte, Emília P. | - |
dc.date.accessioned | 2008-09-01T15:40:54Z | - |
dc.date.available | 2008-09-01T15:40:54Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Free Radical Biology and Medicine. 39:12 (2005) 1611-1619 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5358 | - |
dc.description.abstract | Heme oxygenase-1 (HO-1) has been strongly highlighted because of its induction in many cell types by toxic stimuli, including oxidative stress. The intense HO-1 immunostaining in the substantia nigra of Parkinson disease (PD) patients suggests its involvement in the pathogenesis of this neurodegenerative disease. In this work we investigated HO-1 expression in rat substantia nigra postnatal cell cultures under conditions mimicking dopamine toxicity and its modulation by glial cell line-derived neurotrophic factor (GDNF), a potent neuroprotective factor for dopaminergic neurons. In neuron-glia cultures, we found that H2O2, a product of dopamine metabolism, or l-3,4-dihydroxyphenylalanine (l-DOPA), the dopamine precursor used in the therapy of PD, induced a fast up-regulation of HO-1 mRNA and protein levels, followed by a secondary down-regulation. H2O2 and l-DOPA also increased HO-1 expression in astrocyte cultures, but with a delayed time course in H2O2-treated cultures. HO-1 expression was decreased in neuron-glia cultures under conditions under which GDNF up-regulation was observed. Because exogenously applied GDNF prevented HO-1 up-regulation in cultures treated with H2O2 or l-DOPA, and antibody neutralization of GDNF prevented the secondary HO-1 down-regulation observed in neuron-glia cultures, we propose that GDNF negatively modulates HO-1 expression induced by oxidative stress. To our knowledge, this is the first report showing the modulation of HO-1 expression by GDNF. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T38-4GY83GS-1/1/db5c1961511769a30a115fcbfcd902cf | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Astrocytes | en_US |
dc.subject | Dopaminergic neurons | en_US |
dc.subject | Glial cell line-derived neurotrophic factor | en_US |
dc.subject | Heme oxygenase-1 | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Parkinson disease | en_US |
dc.subject | Free radicals | en_US |
dc.title | GDNF modulates HO-1 expression in substantia nigra postnatal cell cultures | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.freeradbiomed.2005.08.005 | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-9300-3523 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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file600ee3246024499fae7f6752d17f1749.pdf | 307.54 kB | Adobe PDF | View/Open |
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