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https://hdl.handle.net/10316/5289
Title: | Elimination of glucose contamination from adipocyte glycogen extracts | Authors: | Nunes, Patricia M. Carvalho, Eugénia Jones, John G. |
Keywords: | Adipocyte; Glycogen extraction; Glucose oxidase; Amyloglucosidase | Issue Date: | 2008 | Citation: | Carbohydrate Research. 343:9 (2008) 1486-1489 | Abstract: | Glycogen was quantified in rat adipocytes by isolation using conventional KOH digestion and ethanol precipitation, followed by hydrolysis and spectrophotometric assay of the glucose product. A concentration of 0.193 ± 0.020 [mu]mol glucosyl units/106 cells was recorded. When this procedure was modified by including a 4 h incubation with glucose oxidase prior to glycogen hydrolysis, the glycogen concentration was found to be 0.055 ± 0.008 [mu]mol glucosyl units/106 cells. Therefore in adipocytes, conventional glycogen assays give substantial overestimates due to incomplete removal of glucose during glycogen isolation. Contaminant glucose can be scavenged in a simple manner by incubation with glucose oxidase prior to glycogen hydrolysis. | URI: | https://hdl.handle.net/10316/5289 | DOI: | 10.1016/j.carres.2008.04.004 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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